两种转录因子对抗性微调MIR1863a以平衡米的阻力和产量特征
Zhang-Wei Hu1, Jun-Hua Wang1, Xiao-Yu Xiong1
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.
Plant biotechnology journal
|July 29, 2025
概括
米微RNA (miRNAs) 能够平衡抗病能力和产量. MIR1863a突变增强了无产量损失的抗爆性,由转录因子MADS51和ESR1.1调节.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物遗传学 农作物遗传学
- 植物病理学 植物病理学
背景情况:
- 植物的免疫激活通常会导致与生长的权衡,导致作物产量减少.
- 识别协调疾病耐药性和作物产量的基因对于改善农业至关重要.
- 微RNAs (miRNAs) 正在成为具有平衡抗性和产量潜力的关键调节者.
研究的目的:
- 调查MIR1863a在调节抗爆病耐药性和大米作物产量之间的权衡中的作用.
- 确定控制MIR1863a表达的监管机制及其对收益率特征的影响.
- 探索操纵MADS51-ESR1-MIR1863a途径以改善作物的潜力.
主要方法:
- 在大米中研究MIR1863a的基因突变和过度表达.
- 对MIR1863a表达的转录因子结合和调节的分析.
- 对抗疾病和产量相关特征的表型评估 (例如,恐慌数,种子设置率).
- 在不同加入中对MADS51和ESR1的哈普洛型分析.
主要成果:
- MIR1863a的突变增强了不损害产量的水抗爆能力,增加了恐慌数量.
- MIR1863a的过度表达或突变对种子设置率等产量特征产生了负面影响.
- 作为对病原体入侵的反应,MIR1863a的表达受到MADS51 (激活器) 和ESR1 (抑制器) 的对抗调节.
- 此外,MADS51的突变还增强了抗爆炸性,而不会导致产量损失.
- MADS51和ESR1的不同类型与大米亚种的不同抗病表型相关.
结论:
- 涉及MADS51,ESR1和MIR1863a的监管机制微调miRNA水平,以平衡米病耐药性和产量.
- 这一途径为增强抗病能力和最佳产量的育种作物提供了一个新的目标.
- 这种监管模块的分种特定演变表明,有不同的策略来管理水中抗性-产量权衡.
相关概念视频
Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
Translational Regulation
98
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
98
Co-activators and Co-repressors
7.5K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.5K
Cooperative Binding of Transcription Regulators
6.6K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.6K
Regulation of Expression at Multiple Steps
1.0K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.0K
Transcriptional Regulation: Riboswitches
115
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
115


