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在转化生长因子β1介导激活后,对肉纤维细胞的转录组分析

Taijiang Hou¹, Lin Zhang¹, Liang Zhao¹

¹College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Poultry science

|July 29, 2025

概括

这项研究以肉木乳腺肌肉病在实验室中的模型,揭示了转化生长因子-β1 (TGF-β1) 通过激活纤维细胞和改变基因表达来驱动纤维化,这对于了解疾病发展至关重要.

科学领域:

动物科学动物科学
分子生物学分子生物学
病理学病理学病理学

背景情况:

木质乳房 (WB) 肌病严重影响肉产业,其特点是胸部主要肌肉纤维化.
纤维细胞激活和细胞外基质 (ECM) 沉积是WB的关键病理特征.
转化生长因子-β1 (TGF-β1) 是纤维细胞激活中的关键细胞因子,但其在WB病原发生中的确切作用尚不清楚.

研究的目的:

使用TGF-β1刺激建立一个体外肉纤维细胞激活模型.
为了研究TGF-β1诱导的纤维细胞激活过程中的转录组变化.
为了确定关键的分子参与者在WB肌肉病的发展.

主要方法:

通过TGF-β1以剂量和时间依赖的方式刺激初级纤维细胞.
纤维细胞转变为肌纤维细胞转变 (FMT) 标记物的表达 (α-SMA,原I,ACTA2,COL1A1,FN1) 被量化.
进行RNA测序 (RNA-seq) 来分析差异表达的信使RNA (DEmRNA).
蛋白质与蛋白质相互作用网络被构建用于识别枢纽基因.

主要成果:

TGF-β1 显著上调 FMT 标记物以剂量和时间依赖的方式 (P < 0.05).
RNA-seq在刺激和控制纤维细胞之间确定了1532个差异表达的mRNA.
确定了关键的上调枢纽基因 (例如AMPD3,PDE10A,PDE4D) 和下调枢纽基因 (例如NT5C1B,PDE1A,ADSL).

结论:

这项研究成功建立了一个体外模型,用于研究肉中TGF-β1诱导的纤维化.
转录组分析揭示了与纤维细胞激活相关的显著分子变化.
这项研究为了解WB肌肉病变的发病机制和TGF-β1诱导纤维化的机制提供了一个框架.

关键词:

不同化的差异化纤维母细胞细胞是如何形成的肌纤维母细胞细胞.在TGF-β1中.转录组转录组就是一个转录组.

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