在状动物中PCNA的双重遗产:保存的复制支架和谱系特定的基因组架构师
Hongzhen Jiang1, Haicheng Li1, Chundi Wang2
1MOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao 266237, China.
European journal of protistology
|July 29, 2025
概括
尽管有序序差异,但纤毛动物中的增殖细胞核抗原 (PCNA) 保持了DNA过程的关键结构特征. 酸PCNA在这些不寻常的生物体中显示出基因组可塑性和稳定性的独特适应性.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 单核细胞生物学 单核细胞生物学
背景情况:
- 增殖细胞核抗原 (PCNA) 对于DNA复制,修复和真核生物的表观遗传调节至关重要.
- 类动物,凭借其独特的核二态和基因组重塑,为研究PCNA的功能适应性提供了一个新的系统.
- 细胞生物学涉及复制合DNA消除和宏核放大等复杂过程,突出显示PCNA在基因组稳定性和可塑性中的作用.
研究的目的:
- 通过遗传学,结构和功能分析,系统地比较状动物和人类PCNA.
- 为了研究PCNA在不同真核细胞系的保存和分离特征.
- 探索PCNA在毛动物中的功能性创新,特别是与它们独特的基因组过程相关.
主要方法:
- 对PCNA序列的遗传学分析.
- 使用AlphaFold3预测的结构分析对Tetrahymena thermophila,Euplotes eurystomus和Stylonychia lemnae进行了预测.
- 基于现有文献和比较基因组学的功能分析.
主要成果:
- 状PCNA与哺乳动物PCNA的序列差异约为50%.
- 在状PCNA结构中发现了保存的三元化接口和伴侣结合动机.
- 阿尔法Fold3模型证实了结构保护尽管序列分歧.
- 功能性见解揭示了表观遗传调节,paralog专业化和状动物的复制带组合中的谱系特异性创新.
结论:
- 酸PCNA保留了在真核生物中保存的基本结构和功能元素.
- 状动物中的PCNA已经发展出独特的适应性来管理它们复杂的基因组动态.
- 在像状动物这样的非模型生物体中研究PCNA为基因组生物学和蛋白质复合体进化的基本原则提供了宝贵的见解.
相关概念视频
01:24Nucleoid
172
The nucleoid represents a structurally and functionally distinct region within prokaryotic cells, where the cell's DNA and associated proteins are housed. Unlike eukaryotic cells, prokaryotes lack a membrane-bound nucleus, and the nucleoid facilitates the organization and accessibility of the genetic material within this constraint. The DNA in most bacteria and archaea exists as a single, circular, double-stranded molecule that is highly compacted through supercoiling and interactions with...
172
01:29Cytoskeletal Proteins in Bacteria
3.5K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.5K
02:38S-Cdk Initiates DNA Replication
4.8K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.8K
02:30Histone Variants at the Centromere
4.5K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.5K
03:01The Replisome
34.8K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
34.8K
02:40Chromosome Structure
23.5K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
23.5K