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基于膜融合和核酸纳米结构的细胞外囊 miRNA 检测

Jixuan Han¹, Xiaoran Liu², Ying Yan²

¹CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, 100190, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing, 101408, China; Sino-Danish Centre for Education and Research, Beijing, 101408, China.

Biosensors & bioelectronics

|July 29, 2025

概括

一种新的测定检测出细胞外囊泡微RNAs (EV-miRNAs) 用于癌症诊断. 这种方法提供了灵敏的检测,并显示了区分乳腺癌患者和健康个体的临床潜力.

科学领域:

生物化学生物化学
分子生物学分子生物学
纳米技术纳米技术

背景情况:

细胞外囊泡微RNAs (EV-miRNAs) 是癌症进展和耐药性的关键指标.
目前用于EV-miRNA检测的方法受到低丰度和复杂程序的限制,阻碍了临床应用.

研究的目的:

开发一种用于检测EV-miRNAs的新,敏感和高效的测试方法.
评估开发的试验对乳腺癌诊断和治疗监测的临床实用性.

主要方法:

开发了一种结合DNA介导膜融合 (DMF) 和DNA四面体 (DT) 与催化发针组装 (CHA) 的单步测试 (DMF-DT-CHA).
该测试利用DNA相互作用将探针送入EV,然后通过miRNA识别和光信号放大通过CHA.
分析了来自乳腺癌细胞系的EV,并将结果与定量PCR (qPCR) 进行比较.

主要成果:

通过DMF-DT-CHA测定,EV-miRNAs的检测极限达到0.24 fM.
临床评估证明了该试验在区分乳腺癌患者与健康捐赠者 (AUC=0.850) 和良性乳腺结节患者 (AUC=0.835) 中的有效性.
该试验在预测三阴性乳腺癌的治疗反应方面显示了81.1%的准确性.

结论:

DMF-DT-CHA测定提供了一种敏感且高效的EV-miRNA检测方法.
这一平台具有重要的临床潜力,用于癌症诊断和治疗监测,特别是乳腺癌.

关键词:

癌症癌症癌症癌症细胞外囊泡细胞外囊泡膜融合是什么? 膜融合是什么?核酸探测器的核酸探测器信号放大信号放大这是一个小RNARNA.

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