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miR-423-3p通过对GPX4的负调节来缓解sevoflurane诱导的学习和记忆功能障碍以及神经损伤

Liquan Qiu¹, Licai Zhang¹, Bin Fan¹

¹Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong 643000, China.

Neuroscience letters

|July 29, 2025

概括

在小鼠的下调 miR-423-3p 降低了 sevoflurane 麻醉后的认知缺陷. 这种抑制通过负调节GPX4来减轻氧化应激和炎症,提供了一个潜在的治疗点.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
麻醉学麻醉学

背景情况:

黄麻醉可以导致神经损伤,有改变的微RNA表达,包括miR-423-3p.
在sevoflurane引起的神经损伤中miR-423-3p的特定作用尚未完全理解.

研究的目的:

为了研究miR-423-3p在sevoflurane麻醉后对大鼠的影响.
阐明miR-423-3p对神经损伤的影响背后的分子机制.

主要方法:

定量实时PCR (RT-qPCR) 用于测量大鼠海马中的miR-423-3p,GPX4和氧化应激标志物.
行为测试 (莫里斯水迷宫,新型物体识别) 来评估认知功能.
与酶相关的免疫吸收试验 (ELISA) 用于量化炎症因素.

主要成果:

塞沃氨酸麻醉增加了miR-423-3p,并降低了GPX4表达.
下调的miR-423-3p通过对GPX4进行负调节,改善了认知功能,并减少了氧化应激 (ROS,MDA) 和炎症 (NLRP3,Caspase-1,IL-8,IL-1β).
抑制GPX4可以逆转miR-423-3p下调的保护作用.

结论:

下调miR-423-3p减轻了暴露于sevoflurane的老鼠的认知缺陷和神经损伤.
抑制的miR-423-3p减轻氧化应激和炎症,主要是通过对GPX4的负调节.

关键词:

在GPX4中使用GPX4.炎症炎症是一种炎症.氧化应激是一种氧化应激.西沃氨酸是什么?在 miR-423-3p 中使用.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...