在lncRNA roX1中进行性RNA编辑通常是非适应性的
Jiyao Liu1, Qiuhua Xie1, Wanzhi Cai1
1China Agricultural University.
概括
在Drosophila roX1长非编码RNA中,腺素-至-素 (A-to-I) RNA编辑是广泛存在的,但可能不适应. 这项研究没有发现证据表明roX1中的A-to-I编辑在剂量补偿途径中起作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 腺至氨基酸 (A-to-I) RNA编辑是一种关键的转录后修改.
- 编码RNA的A-to-IRNA编辑的适应意义已确立,但对于非编码RNA尚不清楚.
研究的目的:
- 研究Drosophila长非编码RNA (lncRNA) roX1.1中A-to-IRNA编辑的适应意义.
- 确定roX1RNA编辑在剂量补偿途径中的作用.
主要方法:
- 在不同样本中分析Drosophila roX1的A-to-I编辑水平.
- 将roX1编辑水平与基因表达和剂量补偿效率进行比较.
- 在阿达尔突变中对剂量补偿的评估.
主要成果:
- 在Drosophila roX1 lncRNA中观察到广泛的A-to-IRNA编辑.
- 编辑水平显示,尽管roX1表达的差异,但性别特异性很小.
- 在roX1编辑水平和剂量补偿疗效之间没有发现相关性.
- 阿达尔突变在X染色体基因剂量补偿中没有出现缺陷.
结论:
- 在roX1中肆虐的A-to-IRNA编辑不太可能对剂量补偿具有功能意义.
- 在roX1中观察到的编辑似乎没有赋予选择性优势.
- 结果支持分子误差假设,表明适应性变化很少见.
- 在等待进一步的证据之前,在roX1内部不能完全排除适应性个人编辑站点的可能性.
相关概念视频
RNA Editing
9.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.2K
lncRNA - Long Non-coding RNAs
8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K
Types of RNA
6.3K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
6.3K
Non-LTR Retrotransposons
11.8K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.8K
Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
RNA Interference
26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K


