从Enterococcus faecium获得的后生物的探索 HDRsEf1及其益生菌机制
Yingying Chen1, Yingting You1, Lizhen Ren1
1State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Microorganisms
|July 30, 2025
概括
来自Enterococcus faecium (EPS-Ef1) 的耐热外聚糖被确定为一种强大的后生物. 这种成分有效地减少了肠道炎症,并促进了肠道上皮细胞的增殖,提供了新的治疗途径.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 肠球菌 (Enterococcus faecium HDRsEf1) 产生耐热的生物活性化合物.
- 炎症和肠道健康是治疗干预的关键领域.
研究的目的:
- 为了识别来自Enterococcus faecium HDRsEf1.1.的耐热生物活性成分.
- 研究这些成分的抗炎和细胞增殖机制.
- 在体内评估已识别的成分的疗效.
主要方法:
- 从细菌培养超水生物中分离和表征原蛋白 (P-Ef1) 和外聚糖 (EPS-Ef1).
- 使用MODE-K细胞进行体外测试,以评估抗炎作用 (CXCL-1表达) 和细胞增殖 (EdU,PCNA mRNA).
- 在体内研究涉及口服EPS-Ef1给动物,以评估对体重,形形态和肠道炎症的影响.
- 使用HPAEC和SEC-MALLS-RI对EPS-Ef1的结构分析.
主要成果:
- 经热处理的原始EPS-Ef1,但不是原始P-Ef1,通过抑制LPS刺激细胞中的CXCL-1表达,表现出显著的抗炎活性.
- 原始EPS-Ef1促进了MODE-K细胞的增殖,可能是通过PCNAmRNA的上调.
- 在体内口服原始EPS-Ef1增加了体重增加,阴茎密室深度,并降低了肠道CXCL-1mRNA水平.
- 结构分析显示,原始EPS-Ef1是一种由曼诺糖,葡萄糖,葡萄糖酸和银河糖组成的异多聚糖.
结论:
- 来自Enterococcus faecium HDRsEf1的原始EPS-Ef1是一种具有双重有益作用的耐热后生物.
- EPS-Ef1表现出显著的抗炎和肠上皮细胞增殖特性.
- 这些发现为开发EPS-Ef1作为新型后生物治疗剂提供了坚实的理论基础.
相关概念视频
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Probiotics
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
Introduction to the Human Microbiota
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity, and disease...
Development of Human Microbiota
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...
Microbiota of the Large Intestine
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...


