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在饮用水生物膜中对铜腐蚀的转录基因反应
Jingrang Lu1, Ian Struewing1, Nicholas J Ashbolt2
1U.S. EPA Office of Research and Development, Cincinnati, OH 45268, USA.
Microorganisms
|July 30, 2025
概括
铜管道影响饮用水生物膜,改变微生物群落和基因表达. 虽然抑制了一些微生物,但铜增强了其他微生物的抵抗力和特定的代谢途径.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 材料科学 材料科学 材料科学
背景情况:
- 饮用水中的生物膜是复杂微生物群落的宿主.
- 铜 (Cu) 腐蚀对微生物新陈代谢和生物膜中耐药性的影响尚不清楚.
研究的目的:
- 评估上游铜管材料如何影响下游饮用水生物膜中的微生物群落.
- 评估可行的社区结构,病原体种群和转录基因反应的变化.
主要方法:
- 从下游生物膜样本生成和测序随机转录的cDNA.
- 来自未经塑化聚乙烯 (PVC) 和铜 (Cu) 优惠券的比较生物膜.
主要成果:
- 受Cu影响的生物膜显示了丰富的类似病原体和机会性病原体.
- 在Cu生物膜中的上调基因与 (K) 代谢,酸合成,应激反应和毒性化合物耐药性有关.
- 下调的基因包括离子相互作用,呼吸,RNA/代谢,毒性和抗生素耐药性的基因.
结论:
- 微生物生物膜通过改变基因表达,代谢途径和结构组织来适应Cu管道.
- 铜抑制了许多微生物,但促进了饮用水生物膜中的其他微生物的抵抗和特定适应.
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