NOD2 (核酸结合寡聚化域含蛋白2) - - 通过BCG (Bacillus Calmette-Guérin) 诱导的免疫反应的调节
Magdalena Jurczak1,2, Joanna Kaczmarek3, Magdalena Kowalewska-Pietrzak3
1Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology, and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Pathogens (Basel, Switzerland)
|July 30, 2025
概括
卡尔梅特-盖林菌 (BCG) 疫苗通过促进核酸结合性寡合化域含蛋白2 (NOD2) 途径来增强儿童的先天免疫力. 这支持对RSV和SARS-CoV-2等呼吸道病毒的防御.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 传染性疾病 传染性疾病
背景情况:
- 众所周知,Bacillus Calmette-Guérin (BCG) 疫苗可以诱导广泛的非特异性免疫力.
- 核酸结合寡聚化域含蛋白2 (NOD2) 途径对于先天性免疫反应至关重要.
- 单细胞表面受体调节在BCG诱导的免疫力对呼吸道病毒的作用尚不清楚.
研究的目的:
- 研究BCG疫苗接种对儿童先天免疫反应的影响.
- 通过BCG和病毒抗原进行刺激后分析NOD2和相关细胞因子的表达.
- 评估单细胞表面受体对BCG和病毒共同刺激的反应的调制.
主要方法:
- 接种BCG疫苗的儿童的全血培养被活体BCG,SARS-CoV-2或RSV抗原刺激.
- 用RT-qPCR量化NOD2和关键细胞因子的mRNA水平.
- 流细胞计评估了单细胞表面标记物的表达 (CD14,HLA-DR,CD11b,CD206).
主要成果:
- 与BCG和RSV共同刺激显著上调NOD2,IL-1β和IL-6与单独RSV相比.
- SARS-CoV-2 血清阳性捐赠者单独使用RSV显示出更高的NOD2表达,而IL-2 则在BCG + SARS-CoV-2 联合刺激下达到峰值.
- 病毒抗原在单细胞上增加了CD14和HLA-DR的表达,表明激活;CD206的表达仅在双重阳性儿童中升高.
结论:
- BCG刺激通过NOD2相关的细胞因子产生影响儿科抗病毒免疫力.
- BCG诱导CD14+HLA-DR+单细胞表型,这表明免疫活性增强.
- 这些发现支持BCG在增强对呼吸道病原体的天生的防御能力方面的潜在作用.
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