相关实验视频

Updated: Sep 13, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

微型enOsCas12f1使米中的向基因组编辑成为可能

Junjie Wang¹, Qiangbing Xuan¹, Biaobiao Cheng¹

¹College of Life Science, Henan Normal University, Xinxiang 453007, China.

Plants (Basel, Switzerland)

|July 30, 2025

概括

enOsCas12f1的CRISPR系统有效地编辑大米基因,使得有针对性的DNA修改和基因表达增强能够改善作物发育. 这扩大了精确的水基因组工程的可用工具.

科学领域:

农业科学农业科学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

第五型CRISPR/Cas12f系统提供了广泛的PAM识别,小尺寸和易于传输,增强了基因编辑工具箱.
米中的enOsCas12f1系统的活性和特异性以前没有被描述.

研究的目的:

为了研究大米中enOsCas12f1的DNA裂变活性和基因编辑效率.
评估enOsCas12f1对基因修改的能力,包括促进剂和5' UTR编辑,以及在大米中增强基因表达.

主要方法:

在大米原生质中暂时表达,以检测与TTN PAMs的enOsCas12f1活性.
在OSPDS基因中稳定转换大米植物以评估enOsCas12f1基因编辑效率在TTT和TTC PAM.
一个死亡的enOsCas12f1与转录激活域 (TV) 融合,以调节OsIPA1基因表达.

主要成果:

enOsCas12f1表现出DNA裂变活性,识别了大米原生质中的TTN PAMs.
稳定转换揭示了OSPDS基因中EnOsCas12f1诱导的突变 (7-29bp删除),效率为6.21% (TTT PAM) 和44.21% (TTC PAM),导致白化表型.
enOsCas12f1成功编辑了OsDREB1C的促进子和5' UTR,产生了突变的水植物. 修改后的 enOsCas12f1 增强了 OsIPA1 的表达.

结论:

enOsCas12f1是一种功能性基因编辑工具,能够精确修改DNA和调节基因表达.
该系统能够准特定的PAM序列 (TTN,TTT,TTC) 并诱导删除,从而扩大其在米功能基因组学和作物改进中的实用性.
enOsCas12f1扩大了基于CRISPR的米基因编辑工具箱,促进了各种基因修改.

关键词:

基础删除删除 enOsCas12f1 的意思是什么?基因组编辑基因组编辑转录激活的转录激活

更多相关视频

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

相关概念视频

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...