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泰国的禽类甲型肺炎病毒:分子检测,遗传多样性及其对家禽的潜在威胁

Sudarat Wanarat¹, Manakorn Sukmak², Nantana Soda³

¹Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.

|July 30, 2025

概括

泰国家禽中的禽类甲型肺炎病毒亚型B (aMPV/B) 显示出显著的遗传多样性和正在进行的进化. 确定了两种不同的血统,表明自2006年以来的局部传播和多样化,影响了疫苗策略.

科学领域:

兽医病毒学兽医病毒学
禽类的健康禽类的健康
分子流行病学分子流行病学

背景情况:

禽类甲型肺炎病毒亚型B (aMPV/B) 由于呼吸和生殖问题,导致家禽的经济损失很大.
了解aMPV/B的遗传构成和传播对于有效控制商业家禽的疾病至关重要.

研究的目的:

调查泰国aMPV/B的流行,遗传多样性和进化史.
在泰国家禽种群中识别不同的血统及其起源.
根据分子发现,为未来的疫苗接种策略提供信息.

主要方法:

从泰国商业家禽养殖场收集了167个临床样本.
嵌套RT-PCR针对aMPV/B G基因进行检测.
用于遗传多样性评估的遗传学和中间连接网络分析.
对差异时间估计的贝叶斯框架.

主要成果:

发现aMPV/B的流行率为34.13% (57/167).
确定了两种不同的泰国aMPV/B血统,一种与疫苗菌株有关,另一种与新型血统有关.
最近的共同祖先估计在2006年左右,在2009年和2016年左右出现多样化.
在G基因中观察到基因特异性氨基酸替代.

结论:

流通的泰国aMPV/B菌株可能起源于有限的引入,随后是局部进化.
突出了泰国aMPV/B的显著分子异质性和持续的多样化.
建议持续的基因组监测和特定区域的疫苗接种.

关键词:

禽类的甲型肺炎病毒差异时间分析差异时间分析遗传多样性遗传多样性分子检测分子检测检测植物遗传学分析类家禽类家禽这是B型亚型.

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