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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

6.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Sep 13, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

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基于波导的TIRF显微镜中的基于的超分辨率成像 - - 一项实验和数值研究.

Chunyu Lu, Mohammad Talebi Khoshmehr, Mohammad Sadegh Feiz

    Optics express
    |July 30, 2025
    PubMed
    概括

    本研究介绍了一种改进的基于的超分辨率成像 (ESI) 算法,用于全内部反射光 (TIRF) 显微镜. 这种新方法提高了图像的分辨率和精度,克服了现有技术的局限性,以获得更好的高通量成像.

    科学领域:

    • 光学显微镜是一种光学显微镜.
    • 超高分辨率成像成像技术
    • 生物物理学的生物物理.

    背景情况:

    • 光学波导为全内反射光显微镜 (TIRF) 提供了优势,使得视野更大,系统更强大,更紧.
    • 基于的超分辨率成像 (ESI) 具有在没有专门硬件的情况下进行计算图像质量增强的潜力,但未得到充分利用.
    • 现有的ESI实施经常存在缺陷,导致科学结论的结果不可靠.

    研究的目的:

    • 实施和评估一个新的ESI算法用于TIRF显微镜.
    • 确定当前ESI方法的局限性,并评估改进的算法对TIRF应用的适用性.
    • 使用开发的ESI算法,在TIRF成像中展示增强的分辨率和准确性.

    主要方法:

    • 在各种条件下进行模拟,以评估ESI算法的性能和局限性.
    • 化光学波导被制造并涂上TetraSpeck微球以进行实验验证.
    • 开发的ESI算法应用于实验数据,并与现有算法进行比较.

    主要成果:

    • 模拟确定了ESI算法的局限性和适用于TIRF显微镜的适用性.
    • 使用现有的ESI插件进行的初始实验未能提高分辨率,并产生不准确的测量结果.
    • 新的ESI算法成功提高了分辨率,与理论预测一致,并纠正了不准确.

    更多相关视频

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

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    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
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    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

    Published on: March 22, 2012

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    相关实验视频

    Last Updated: Sep 13, 2025

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

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    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

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    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
    16:10

    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

    Published on: March 22, 2012

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    结论:

    • 开发的ESI算法克服了现有方法的局限性,为TIRF显微镜提供了显著的改进.
    • 这项工作验证了新ESI算法的有效性,实现了更高的分辨率和更准确的测量.
    • 改进的ESI算法为高通量和长期TIRF成像应用提供了可靠的计算工具.