抗微生物可以促进全血从细菌安全:一个概念的证明
Supaksorn Chattagul1, Joseph Jackson1, William C Wimley2
1Office of Blood Research and Review, Center for Biologics Evaluation and Research, Silver Spring, Maryland 20993, United States.
ACS infectious diseases
|July 30, 2025
概括
新型抗微生物 (AMP) 在储存期间在全血 (WB) 中防止细菌污染方面表现有前途. 这项研究证明了它们的有效性和安全性,提供了一个潜在的新策略,以提高血液产品的安全性和减少输血相关的风险.
科学领域:
- 生物技术和生物医学科学 生物技术和生物医学科学
- 血液学和输血医学 血液学和输血医学
- 抗菌研究 抗菌研究
背景情况:
- 储存全血 (WB) 的细菌污染仍然是一个罕见但重要的风险,可能导致败血症输血反应和传染性感染.
- 目前的风险减轻策略包括供体查和病原体减少技术 (PRT),但不断寻求新的方法.
- 抗微生物 (AMP) 是一种具有广泛抗微生物活性的分子类别,可以为血液安全提供新的途径.
研究的目的:
- 评估两种新型阴性抗微生物 (AMP),D-CONGA和D-CONGA-Q7在预防全血 (WB) 细菌污染方面的有效性.
- 通过检查它们对WB血液溶解和血静性质的影响,评估这些AMP的安全性.
- 为了证明从WB后处理中去除AMP的可行性.
主要方法:
- 整体血液 (WB) 接种了大肠杆菌和海皮杆菌.
- D-CONGA和D-CONGA-Q7的杀菌疗效使用最小抑制度 (MIC) 和时间杀死动力学测试来确定.
- 在经过化处理和随后使用化交换树脂去除后,在35天的储存期间评估了血液溶解,血液静止性质和细菌生长.
主要成果:
- D-CONGA和D-CONGA-Q7对被测试的细菌表现出强烈的杀菌活性,MIC在1-8μM之间.
- 度为8μM,可以降低细菌负载的5-log10,显示出显著的杀菌效果.
- 20μM的AMP治疗没有对血液溶解或静血性质产生不利影响,而4μM的D-CONGA-Q7在35天的WB储存期间阻止了细菌生长.
结论:
- 新型阴离子AMP,D-CONGA和D-CONGA-Q7,显示出作为全血的主动病原体减少技术的巨大潜力.
- 这些AMP有效地减少了细菌污染,而不会影响WB组件的安全性或完整性.
- 这些发现为使用特定的AMP来提高储存期间血液制品的安全性提供了概念证明.
相关概念视频
Antimicrobial Proteins
5.4K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
5.4K
Surface Membrane Barriers
1.5K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.5K
Biological Methods for Microbial Control
206
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
206
Defense Mechanism Against Infection
7.2K
Natural flora, body system defenses, and inflammation are natural barriers of the body against infectious agents regardless of previous exposure. Normal floras of the human body refer to the microbial population that colonizes the skin and mucous membranes.
In addition, many body organ systems have unique defenses against infection. The skin is an intact, multilayered surface preventing invasion by microorganisms unless impaired. Mucous membranes lining the mouth, nose, and eyelids are barriers...
In addition, many body organ systems have unique defenses against infection. The skin is an intact, multilayered surface preventing invasion by microorganisms unless impaired. Mucous membranes lining the mouth, nose, and eyelids are barriers...
7.2K
Defense Against Bacterial Pathogens
1.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.5K
Bacterial Signaling
34.2K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
34.2K


