评估脱调比率作为一种工具,用于检测LC-MSMS分析中的潜在干扰
Arber Rexhaj1, Michael Vogeser1, Katharina Habler1
1Institute of Laboratory Medicine, LMU University Hospital, LMU Munich, Germany.
差调比 (DR) 可以在质谱 (MS/MS) 分析中检测异构干扰. 该方法补充了离子比 (IR) 检测,提高了临床质谱结果的可靠性.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 临床诊断 临床诊断 临床诊断
背景情况:
- 双重质谱 (MS/MS) 提供高特异性,但易受异构/异构干扰.
- 临床实验室目前使用离子比 (IR) 检测来识别单个样本中的干扰.
- 需要补充方法来提高MS/MS分析的准确性.
研究的目的:
- 实验评估使用差调效应作为检测MS/MS.中的异构干扰的方法.
- 评估脱调比 (DR) 检测用于识别具有相同质量转换的意外物质作为目标分析物的适用性.
主要方法:
- 通过评估仪器设置对分析离子产量的差异影响来确定脱调比 (DR).
- 已知异构干扰物质被添加到目标分析物质中,以量化调查DR检测适用性.
- 两个示例测试系统,科蒂松/普雷尼索隆和O-Desmethylvenlafaxine/cis-Tramadol HCl,被用于评估.
主要成果:
- 观察到脱调比 (DR) 表明在两个示例测试系统中存在异构干扰.
- 在受异构或异构干扰影响的样本中观察到DR的变化.
结论:
- 脱调比 (DR) 检测是一种可行的方法,用于指示液态染色体协同质谱法 (LC-MS/MS) 中的异构或异构干扰.
- 可以将DR检测与已确定的离子比 (IR) 检测一起使用,以提高临床MS分析的分析可靠性.
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