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Updated: Sep 13, 2025

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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在Hansenula polymorpha DL-1中开发精确的基因组编辑和多副本集成工具

Xiaoyi Zou1,2, Jiaqi Miao1,2, Hongbiao Li1,2

  • 1Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China.

Synthetic and systems biotechnology
|July 30, 2025
PubMed
概括

这项研究开发了Hansenula多态酵母的先进合成生物学工具,改进了基因组编辑和基因整合,以可持续生产有价值的化合物.

关键词:
这就是CRISPR-Cas9的特征.汉塞努拉多态植物DL-1同类的重组组合.多复制集成的多复制集成中立网站 中立网站

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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