在发酵过程中的高温Daqu中,物理化学性质和微生物群落的时空动态
Yu-Hua Wei1,2, Zhang Wen1,2, Shang-Jie Yao3
1State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Food chemistry: X
|July 30, 2025
概括
高温达克 (HTD) 发酵显示,在第五天之后,由于显著的物理化学和微生物变化,形成了不同的层. 上层表现出更高的酶活性,已确定代谢物和微生物生物标志物影响了Daqu的形成.
科学领域:
- 食品微生物学 食品微生物学
- 发酵科学 发酵科学
- 生物技术是生物技术.
背景情况:
- 高温达克 (HTD) 是通过堆叠固态发酵至关重要的味Baijiu生产.
- 形成明显的白色,黄色和黑色的达库层在发酵后形成,但基本的机制尚不清楚.
研究的目的:
- 在HTD发酵过程中研究物理化学性质,代谢物和微生物群落的时态空间动态.
- 阐明驱动达库层差异化的机制.
主要方法:
- 在40天内对不同HTD层的物理化学特性进行比较分析.
- 代谢分析分析以识别差异性代谢物.
- 使用16S rRNA测序进行物种级微生物社区分析.
- 构建微生物共发生网络和相关性分析.
主要成果:
- 从第8天到第40天,在HTD层之间观察到显著的物理化学和微生物差异化.
- 上层的Daqu表现出优异的糖化和液化酶活动.
- 确定了39种差异性代谢物和13种微生物生物标志物,与特定层相关.
- 阐明了塑造层特异性社区的关键物理化学因素和微生物相互作用.
结论:
- 在HTD中的层分化是由物理化学和微生物形状的明显时间和空间变化驱动的.
- 酶活动,特定代谢物和微生物社区结构在形成不同类型的Daqu中起着至关重要的作用.
- 这项研究提供了对HTD复杂发酵过程的基础见解.
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