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Updated: Sep 13, 2025

Synthesis of an Intein-mediated Artificial Protein Hydrogel
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Published on: January 27, 2014

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通过N-糖基化共识序列融合域的多功能蛋白质凝的超分子组合

Eric D Hill1, Stephen Michel1, Natasha R Sequeira1

  • 1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Biomedical Sciences J293, PO BOX 116131, 1275 Center Drive, Gainesville, FL, USA.

Molecular systems design & engineering
|July 30, 2025
PubMed
概括

研究人员开发了一种新的N-糖化标签 (NGT),当与蛋白质融合时,形成温度敏感的生物材料. 这一进步使得使用基于细胞的表达系统能够创建功能性多蛋白融合.

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Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
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Oligosaccharide Assembly01:24

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Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
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Globular Proteins

In organisms, proteins are the most abundant macromolecules. They act as the building blocks of life and play various crucial roles in the body. Proteins can be broadly classified into two distinct subtypes based on their shape and solubilities: globular proteins and fibrous proteins.
Globular proteins serve many important physiological functions, such as acting as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be soluble in the aqueous...
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Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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