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使用机器学习解码试类动物的转录后基因表达控制

Michele Tinti¹, David Horn¹

¹The Wellcome Centre for Anti-Infectives Research, Biological Chemistry & Drug Discovery, University of Dundee Division, Dundee, Scotland, UK.

Wellcome open research

|July 30, 2025

概括

试类动物的基因表达是由未翻译区域 (UTR) 和代码子使用偏差控制的,主要影响跨物种的翻译效率和mRNA水平.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
寄生虫学的寄生虫学

背景情况:

在Trypanosoma brucei的mRNA中,未翻译区域 (UTR) 具有cis调节作用.
在UTR中,A丰富的区域与翻译效率 (TE) 的提高相关.
关键问题涉及UTRs,代使用偏差,以及它们对T. brucei,T. cruzi和Leishmania中TE和mRNA水平的影响.

研究的目的:

确定UTRs和密码体使用偏差对T. brucei.的翻译效率 (TE) 的相对贡献.
评估UTR和编码子使用偏差对T. brucei. mRNA平稳状态水平的影响.
为了研究这些序列在TE和mRNA水平中的作用,在相关的试类动物,T. cruzi和Leishmania.

主要方法:

使用了机器学习方法.
分析现有的转录组,翻译效率和蛋白质组学数据.
在三种试类动物中进行了对比分析.

主要成果:

无论是UTRs还是codon使用偏差,都会影响三种试类动物的基因表达,具有特定物种的差异.
在T. brucei中,TE与较长的A丰富和C贫乏的UTR相关.
的使用偏差显著影响所有物种的mRNA丰度,可能是通过翻译延长率.

结论:

试类动物的基因表达控制主要是转录后的,在翻译水平.
UTRs影响翻译启动率.
受青的编码子增强了翻译延长,减少了mRNA循环,增加了稳定性.

关键词:

在Codon Bias中存在偏见.莱什曼尼亚 (Leishmania) 是一种病.机器学习机器学习翻译效率翻译效率试杆菌体是什么?在 UTRs 中使用.

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