来自Listeria monocytogenes菌体的Endolysin LysP70的抗菌活性
Kunzhong Zhang1, Xuehui Zhao1, Qing Cao1
1College of Veterinary Medical, Gansu Agricultural University, Lanzhou, China.
Frontiers in microbiology
|July 30, 2025
概括
一种新型的内素,LysP70,有效地准并消除危险的病原体Listeria monocytogenes. 这种抗菌剂在食品安全和控制细菌感染方面表现有前途.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 恩多利辛是菌素衍生的蛋白质,具有针对格拉姆阳性细菌的抗微生物特性.
- 李斯特菌对食品安全和公共健康构成重大威胁.
- 需要新的抗微生物策略来对抗细菌感染和生物膜.
研究的目的:
- 克隆,表达和净化来自Listeria菌体P70.70的内溶酶LysP70.
- 评估LysP70.0.的抗菌功效和酶特性.
- 评估LysP70在食品应用和生物膜控制中的潜力.
主要方法:
- 基因克隆,蛋白质表达和LysP70.0.的净化
- 使用平板计数和电子显微镜进行抗菌活性评估.
- 在各种pH值,温度和金属离子条件下进行酶稳定性测试.
- 生物膜抑制和根除试验.
- 在牛奶样本中量化L. monocytogenes.
主要成果:
- P70是一种34.2kDa的蛋白质,具有结构稳定性和酶活性.
- 莱斯P70对Listeria monocytogenes表现出特定的性活性.
- 在广泛的条件下观察到稳定的酶活性.
- 莱斯P70有效地抑制和消灭了L. monocytogenes生物膜.
- 牛奶中的L. monocytogenes减少了1.9 Log10 CFU/mL. 这一结果是可以实现的.
结论:
- 莱斯P70是一种强大的抗菌剂,可以对抗Listeria monocytogenes.
- 莱斯P70显示出在食品中控制L. monocytogenes的显著潜力.
- 这项研究为开发基于内素的抗微生物药物提供了基础.
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