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珀瓦里蜂蜜对SH-SY5Y神经母细胞瘤细胞的抗增殖和亡作用

Pınar Altin-Celik¹, Muazzez Derya-Andeden², Hamiyet Eciroglu-Sarban³

¹Department of Medical Biology, Medical Faculty, Erciyes University, 38030, Kayseri, Türkiye. pnar.altnclk@gmail.com.

Medical oncology (Northwood, London, England)

|July 30, 2025

概括

珀瓦里蜂蜜 (PH) 通过降低癌细胞活力和诱导亡,显示出显著的抗神经母细胞瘤作用. 这种天然抗氧化剂显示出作为对儿科神经母细胞瘤的补充疗法的前景.

科学领域:

在瘤学瘤学.
药理学药理学是指药理学的学科.
自然产品化学化学

背景情况:

神经母细胞瘤是一种流行的儿科癌症,治疗选择有限,经常导致氧化应激.
自然抗氧化剂因其有益的特性而被用于化疗和化疗.
珀瓦里蜂蜜 (PH) 是来自土耳其的独特蜂蜜,具有已知的抗氧化和抗炎作用等药用特性.

研究的目的:

研究Pervari蜂蜜 (PH) 对SH-SY5Y人类神经母细胞瘤细胞的体外抗增殖,抗瘤,亡和炎症作用.
评估PH对参与细胞死亡,炎症和细胞外矩阵重塑的关键基因的影响.

主要方法:

使用MTT测定来评估细胞活力.
细胞亡通过Annexin V-FITC/7-AAD染色来定量.
使用RT-qPCR分析了IL-1β,IL-6,TNF-α,NF-κB,Caspase-3,MMP-2,MMP-9,BAX和BCL-2的基因表达.使用RT-qPCR进行了分析.

主要成果:

在剂量和时间上,PH显著降低了神经母细胞瘤细胞活力.
通过上调调节亲细胞亡的BAX和Caspase-3和降低抗细胞亡的BCL-2来诱导PH诱导的细胞亡.
PH调节的炎症性细胞因子 (IL-1β,IL-6,TNF-α) 和增加的NF-κB表达,以及增加的MMP-2和MMP-9水平.

结论:

珀瓦里蜂蜜在体外对神经母细胞瘤细胞具有显著的抗瘤潜力.
PH表现出免疫调节性质,并影响细胞外矩阵重塑.
进一步的体外和体内研究是有必要的,以探索PH在神经母细胞瘤治疗中的治疗应用.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.细胞活力细胞活力炎症炎症是一种炎症.佩尔瓦里蜂蜜 (Pervari honey) 是一种蜂蜜.在SH-SY5Y神经母细胞瘤细胞中.

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