动物菌 (Macrococcus animalis) sp. 这种病原体 nov. 和 Macrococcus equi sp. 的不同. nov.,从不同的动物来源中分离出来
Chahrazed Belhout1, Fangkun Wang1,2, Alexandra Rossano1
1Division of Molecular Bacterial Epidemiology & Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
概括
从动物样本中鉴定出了两种新的格拉姆阳性细菌物种Macrococcus equi和Macrococcus animalis. 这些新物种与已知的Macrococcus物种不同,基于基因组和化学分类学分析.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 基因组学就是基因组学.
背景情况:
- 麦克罗科克 (Macrococcus) 属包括在与动物相关的环境中发现的格拉姆阳性菌.
- 准确的分类学分类对于理解微生物多样性和潜在的作用至关重要.
研究的目的:
- 从各种动物材料中分离出来的新型细菌菌株的特征和分类.
- 用多相分类学来划分Macrococcus属内的新物种.
主要方法:
- 使用全基因组序列和数字DNA-DNA杂交 (dDDH) 的类基因组学分析.
- 16S rRNA,dnaJ,hsp60,rpoB 和 sodA 基因序列的比较.
- 化学分类学分析,包括极性脂质分析和脂肪酸分析.
- MALDI-TOF MS光谱分析和表型表征.
主要成果:
- 九个菌株在遗传学上被放置在Macrococcus caseolyticus分类中,与现有的物种密切相关.
- 基因组数据 (dDDH <24%,ANI <82%) 支持分类两个不同的新物种.
- 麦克罗科克斯 (Macrococcus equi sp.) 已经成为一种常见的病菌. nov. (类型菌株18KM445T) 和动物菌 sp. 新的 (型株EM39ET) 已被提出.
- 新的物种可以通过12.5%NaCl的生长和特定的代谢活动来区分.
结论:
- 根据综合的基因组,化学分类学和表型学数据,提出了两种新物种Macrococcus equi和Macrococcus animalis.
- 这些物种扩大了Macrococcus属内已知的多样性.
- 这些发现有助于准确分类与动物相关环境相关的细菌.
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