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在模拟阳光支持的反扩散细菌中减少抗生素和抗生素耐药性基因-藻类生物膜:接口特性和功能基因反应

Han Zhang¹, Jiao Yin¹, Haotian Jiang¹

¹State Key Laboratory of Urban-rural Water Resource and Environment (SKLUWRE), Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, PR China.

|July 30, 2025

概括

一种新型的细菌-藻类系统 (UV-MABAR) 在模拟阳光下有效降解抗生素残留物并减少抗生素耐药性基因 (ARG). 这种共生方法维持了微生物的健康,与非藻类系统不同,防止了污染物的积累.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
处理水处理水处理水处理

背景情况:

抗生素残留物和抗生素耐药性基因 (ARG) 构成重大环境和健康风险.
传统的废水处理方法难以同时有效地去除抗生素污染物和ARG.
生物膜系统具有微生物修复的潜力,但可以受到UV辐射等环境压力因素的负面影响.

研究的目的:

设计和评估一种新的细菌-藻类共生反扩散生物膜系统 (UV-MABAR),用于同时去除抗生素残留物和ARG.
研究藻类在模拟太阳辐射下对生物膜的稳定性和功能有所贡献的机制.
在污染物清除和微生物群体完整性方面,比较UV-MABAR系统与非藻类控制 (UV-MABR) 的性能.

主要方法:

在模拟的阳光下开发UV-MABAR系统,将细菌和藻类集成到反扩散生物膜配置中.
使用UV-MABR (非藻类控制) 进行生物膜性能比较分析.
量化了抗生素 (硫甲醇 - SMX) 度,ARG表达 (sul1,sul2),微生物基因表达 (recA,ATP合成酶,DNA修复) 和废水参数 (NH4+-N,总,COD).
对光火和形状变化进行分析的细胞外聚合物 (EPS).

主要成果:

与对照组相比,UV-MABAR系统实现了SMX的70.75%的非生物光降解,并显著降低了sul1和sul2的表达.
非藻类UV-MABR系统显示,生物膜恒温被破坏,导致SMX废水积累 (138.11±2.34μg/L).
紫外线MABAR通过减弱辐射诱导的形状变化和调高细菌reCA表达来维持生物膜完整性,从而在150天内显著降低了废水SMX (19.84μg/L),NH4+-N (3.88 mg/L),总 (12.76 mg/L) 和COD (34.97 mg/L).

结论:

细菌-藻类共生UV-MABAR系统是一种高效的方法,可以同时去除抗生素残留物和ARG.
藻类的整合对于维持生物膜稳定性和微生物社区在紫外线照射下功能至关重要,防止污染物积累.
这种新型系统展示了先进的废水处理应用的巨大潜力,解决了制药和抗菌素耐药性带来的关键环境挑战.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.生物膜的反应反应.反扩散的细菌藻类生物膜.宏观转录组 (macrotranscriptome) 是一个宏观转录组.模拟太阳光照射的模拟太阳光

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