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计算IL-37b变体的设计,以破坏二元化并增强单体稳定性

Shiyu Hu¹, Fanyu Zhao¹, Jian Wang²

¹NYU-ECNU Center for Computational Chemistry and Shanghai Frontiers Science Center of Artificial Intelligence and Deep Learning, NYU Shanghai, Shanghai 200124, China; Department of Chemistry, New York University, New York, New York 10003, USA.

International journal of biological macromolecules

|July 30, 2025

概括

研究人员设计了Interleukin-37b (IL-37b) 变体,以改善抗炎疗法. 这种蛋白质工程方法成功地减少了二分化和增强了单体稳定性,为更有效的治疗铺平了道路.

科学领域:

生物化学和分子生物学
免疫学免疫学免疫学
计算生物学计算生物学

背景情况:

介素-37b (IL-37b) 显示出治疗炎症性疾病的前景.
IL-37b的治疗潜力受到其形成二元体的倾向及其活性单体形式的不稳定性所限制.

研究的目的:

使用in silico蛋白质工程设计具有减少二元化和改善单体稳定性的新型IL-37b变体.
为了确定增强IL-37b抗炎功效和临床功效的特定突变.

主要方法:

在蛋白工程中使用,包括双氨酸扫描和计算站点和突变发生.
采用分子动力学 (MD) 模拟和结构分析来评估变体稳定性和二元化倾向.
确定了对二聚体形成至关重要的热点残留物,并评估了突变对单聚体稳定性的影响.

主要成果:

鉴定了36种有效阻止IL-37b二元体形成的突变.
发现了10种突变,可以增强单体IL-37b的稳定性.
证明突变对二分化和稳定性的影响是独立的,允许结合策略.

结论:

成功设计了具有增强单体稳定性和减少二元化的IL-37b变体.
开发的in silico方法为细胞因子和其他蛋白质的合理蛋白质设计提供了可概括的框架.
这些发现为开发更有效的基于IL-37b的治疗方法提供了有希望的策略.

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