通过Pilus与Lactobacillus crispatus进行联合聚合,通过干扰微殖民地形成,增加了脑膜炎球菌对抗菌剂的敏感性
Kenny Lidberg1, Sarah Pilheden1, Samuddi Nawarathne1
1Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, SE-10691, Sweden.
BMC microbiology
|July 30, 2025
概括
乳酸菌结合于Neisseria meningitidis pili,破坏细菌微殖民地并增加对抗菌素的敏感性. 这种相互作用表明,L. crispatus在对脑膜炎球菌感染的宿主防御中起着有益的作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 宿主-病原体相互作用
背景情况:
- 脑膜炎菌 (Neisseria meningitidis) 无症状地殖民鼻,但可以导致严重的侵入性疾病,如脑膜炎和败血症.
- 鼻粘膜含有共生细菌,包括乳酸菌种,这些细菌可能与病原体相互作用.
- 了解这些相互作用对于开发新的治疗策略至关重要.
研究的目的:
- 为了研究乳杆菌物种和Neisseria meningitidis之间的相互作用.
- 确定细菌 pili 在这种相互作用中的作用.
- 评估这种相互作用对脑膜炎球菌毒性和宿主防御的影响.
主要方法:
- 在不同Lactobacillus菌株和N. meningitidis之间进行协同聚合测试.
- 对N. meningitidis pili (I和II类) 与L. crispatus.的相互作用进行分析.
- 评估微殖民地形成,通过抗菌剂杀死细菌 (LL-37,hBD2,塞法莱克辛) 和细菌运动.
主要成果:
- 乳杆菌crispatus菌株MV24与N. meningitidis. 进行特定的联合聚合.
- 这种联合聚合是由L. crispatus与N. meningitidis pili.结合的媒介.
- 同聚会破坏了脑膜炎球菌微殖民地形成,增强了抗微生物药物的杀死,并阻碍了细菌的运动.
结论:
- N. meningitidis pili与L. crispatus相互作用,导致对脑膜炎球菌殖民和毒性的显著干扰.
- L. crispatus MV24通过调节N. meningitidis的行为,在宿主防御中发挥了潜在的有益作用.
- 这些发现突显了利用共生细菌控制侵入性脑膜炎球菌疾病的治疗潜力.
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