相关实验视频
Updated: Sep 13, 2025

08:57
Aip1p Dynamics Are Altered by the R256H Mutation in Actin
Published on: July 30, 2014
8.1K
使用CTP对hCTPS1进行丝状
Chen-Jun Guo1, Xiaojie Bao1, Ji-Long Liu2,3
1School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Cell & bioscience
|July 30, 2025
概括
丁三酸盐 (CTP) 合成酶1 (CTPS1) 形成由其产物CTP调节的纤维. 这项冷EM研究揭示了CTPS1调节的保存机制,为癌症和免疫疾病提供治疗点.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 氨酸三酸盐 (CTP) 合成酶 (CTPS) 对于核酸合成和细胞增殖至关重要.
- 人类CTPS1 (hCTPS1) 对免疫反应至关重要,并且在增殖细胞中高度表达,表明其作为治疗点的潜力.
- 对于hCTPS1活动的调控机制尚不清楚.
研究的目的:
- 研究人类CTPS1 (hCTPS1) 活动的调节机制.
- 阐明 CTP 在 hCTPS1 线程和调节中的作用.
- 了解hCTPS1.1中CTP结合和光线组件的结构基础.
主要方法:
- 低温电子显微镜 (cryo-EM) 用于确定CTP结合的hCTPS1丝的结构.
- 生物化学分析分析CTP的监管作用.
- 跨物种的CTP结合口袋的比较分析.
主要成果:
- CTP 作为 hCTPS1 线程的关键调节器.
- 高分辨率的冷电磁结构揭示了CTP结合和丝组装的分子细节.
- 在酶活性过程中产生的CTP不会诱导导光纤分解,这表明保留了调节模式.
- 丝机制在真核CTPS中是进化保守的.
结论:
- 通过线程,CTP结合调节hCTPS1的活动.
- 这项研究揭示了新的,对代谢酶的保护性调节机制.
- 研究结果为开发针对免疫相关疾病和癌症的向治疗提供了洞察力,通过向hCTPS1.
相关概念视频
Assembly of Cytoskeletal Filaments
21.4K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
21.4K
Disassembly of Intermediate Filaments
2.1K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.1K
Generation of Straight or Branched Actin Filaments
3.0K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
3.0K
Mechanism of Filopodia Formation
2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
Catenins
2.4K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.4K
Actin Filament Depolymerization
3.2K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
In F-actin, the ADF/cofilin proteins...
3.2K

