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在细胞表面上可编程的蛋白质结合

Christian Kofoed¹, Girum Erkalo¹, Nicholas E S Tay¹

¹Department of Chemistry, Princeton University, Princeton, NJ, USA.

|July 31, 2025

概括

科学家们开发了一种新的分子装置, 这种可编程系统精确地准细胞,使局部蛋白质产生用于治疗或诊断.

科学领域:

生物化学
分子生物学
细胞生物学

背景情况:

细胞表面景观因细胞类型而异,并且在疾病中发生变化.
目前的策略通常针对单一的表面抗原,限制了特异性.
需要能够集成多个细胞表面特征的可编程系统.

研究的目的:

开发一个自主决策装置,用于在细胞表面产生向蛋白质.
创建一个可编程的分子系统,将多个细胞表面特征集成为用户定义的输出.

主要方法:

使用近距离关闭的蛋白质转接,从不活跃的片段生成活跃的蛋白质.
开发了一种用于细胞表面融合蛋白质结合的蛋白质执行平台.
对各种输入,准方式和输出进行了模块化和调整.

主要成果:

在指定细胞表面实现高度选择性的活性蛋白质生成.
显示生成的蛋白质可以与细胞结合或释放.
已确认与多种输入,定位策略和功能输出的兼容性.

结论:

开发的蛋白质执行器平台可以对细胞系统进行局部查询和操纵.
这种可编程的分子系统为基于细胞的诊断和治疗提供了多功能工具.
这项技术可以基于细胞表面识别来精确控制蛋白质的生成.

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