腹微生物群衍生的细胞外囊泡通过miR-125b/NF-κB介导的巨细胞两极化驱动肠道平衡功能障碍
Mengzhen Song1, Wenjun Zhou1, Jinping Fan1
1College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan City, Hubei, China.
Gut microbes
|July 31, 2025
概括
来自腹肠道微生物的细胞外囊泡 (EV) 促进炎症和肠道屏障损伤. 这些EV中的特定微RNA (miR-125b) 驱动了这一过程,为炎症性肠道疾病提供了潜在的治疗点.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 由肠道微生物群衍生的细胞外囊泡 (EVs) 介导宿主-微生物通信.
- 电动汽车在腹疾病中的作用尚不清楚.
研究的目的:
- 为了研究肠道微生物群衍生的EVs在腹中的致病作用.
- 通过确定EVs导致肠道炎症和屏障功能障碍的分子机制.
主要方法:
- 从腹捐赠体中分离和描述EVs.
- 使用常规和伪无细菌小鼠进行体内研究.
- 的miRNA测序和功能验证miR-125b.
- 评估巨细胞两极分化和肠上皮质屏障的完整性.
主要成果:
- 来自腹微生物群的EV诱导了亲炎性巨细胞两极分化,并损害了肠道屏障的完整性.
- miR-125b在腹EV中具有高度丰富性,并准NF-κBIA,激活NF-κB通路.
- EVs-miR-125b促进了巨细胞炎症,并破坏了肠道紧密的结节,增加了透性.
- 收养转移miR-125b暴露的巨细胞模仿屏障功能障碍.
结论:
- 来自肠道微生物群的EVs,特别是通过EVs-miR-125b,通过促进炎症和破坏肠道平衡,在腹疾病中发挥着重要作用.
- EVs代表了炎症性肠道疾病的潜在生物标志物和治疗点.
- 这项研究强调了EVs携带的miRNAs在王国间通信和宿主免疫力中的重要性.
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