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感染不同牙周病原体的DPSC的细胞分化

Tiansong Xu^1,2, Xiaofen Chai¹, Wen Zhang^3,4

¹Central Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.

|July 31, 2025

概括

卡普诺细胞菌 (Capnocytophaga periodontitidis) 和 Porphyromonas gingivalis 不同地影响牙髓干细胞 (DPSC). C. periodontitidis诱导了类似光滑肌肉的分化,而P. gingivalis促进了矿化,影响了牙周疾病的治疗.

科学领域:

口腔生物学口腔生物学
干细胞研究的研究.
微生物学微生物学

背景情况:

牙髓干细胞 (DPSC) 对于牙再生至关重要.
牙周病可能涉及内牙并发症,影响DPSCs.
了解病原体与DPSC的相互作用对于治疗至关重要.

研究的目的:

调查Capnocytophaga periodontitidis和Porphyromonas gingivalis对DPSC子集群的影响.
分析与分化,缺氧和细胞周期相关的基因表达变化.

主要方法:

从健康的捐赠者中隔离和培养DPSC.
DPSCs感染C.牙周炎或P.牙周炎.
高通量单细胞RNA测序用于分析基因表达.

主要成果:

确定了四个DPSC集群:古典类,纤维细胞类,光滑肌类和周围血管类.
C. periodontitidis诱导了类似光滑肌肉的细胞分化.
P. gingivalis促进了纤维细胞 (矿物化) 谱系,对DPSC分化具有更显著的整体影响.
一个独特的DPSC子集群对C. periodontitidis作出反应,改变了B细胞激活,肌肉增殖和ROS通路.

结论:

在感染C. periodontitidis和P. gingivalis时,DPSC表现出同质性和异质性.
每种细菌都会触发不同的分化途径 (平滑肌肉与矿物化).
这些发现有助于理解和开发治疗性策略,治疗牙周病的牙周内参与.

关键词:

它们是 Porphyromonas gingivalis.卡普诺细胞虫的周期性牙.牙髓干细胞是牙髓的干细胞.牙周病是一种牙周病.一个单细胞RNA测序.

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