更年期前后的不同宫微生物组和代谢物概况:对宫癌进展的影响
Rie Kawasaki1,2, Iwao Kukimoto3, Eiji Nishio2
1Department of Gynecology, Fujita Health University, School of Medicine, Toyoake, Aichi, Japan.
Frontiers in cellular and infection microbiology
|July 31, 2025
概括
更年期改变了宫微生物组,影响了宫癌的进展. 年轻女性和年长女性之间的细菌和代谢变化有所不同,这表明子宫癌的年龄特定个性化医疗方法.
科学领域:
- 妇科瘤学 妇科瘤学
- 微生物组研究 微生物组研究
- 代谢学 代谢学 代谢学
- 免疫学 免疫学 免疫学
背景情况:
- 子宫癌主要是由持续高风险的人类乳头瘤病毒 (HPV) 感染引起的,是妇女的重大全球健康问题.
- 在更年期期间,子宫膜微生物组的组成会有动态变化,这可能会影响子宫瘤的发展和进展.
- 主体的免疫反应,遗传倾向和生活方式因素也会导致宫癌的病因.
研究的目的:
- 调查宫微环境变化与宫瘤进展与更年期状态之间的关联.
- 整合宫微生物组,其相关代谢物,细胞因子和microRNAs (miRNAs) 的分析,以了解它们在宫瘤中的作用.
- 为潜在的个性化治疗策略阐明这些因素的年龄相关差异.
主要方法:
- 一组248名患有宫瘤的HPV阳性女性 (CIN1-3,SCC) 和48名HPV阴性健康对照被按更年期状态分层 (平均年龄50岁).
- 用16S rRNA基因测序分析了宫粘液样本,用于微生物群的概况,超HPLC-tandem质谱用于代谢物分析,以及RT-qPCR用于miRNA表达.
- 量化了细胞因子水平 (RANTES),并使用微生物组分析仪和代谢分析仪分析了数据.
主要成果:
- 在患有状细胞癌 (SCC) 的年轻女性 (例如, *Prevotella*, *Atopobium*) 与老年女性 (例如, *Peptoniphilus*, *Fusobacterium*) 之间,存在不同的细菌系.
- 在CIN2或更严重的年轻女性中观察到*Atopobium*和香之间存在显著的正相关性,但在老年女性中没有.
- *Atopobium*, *Adlercreutzia* 和 *Gardnerella* 在较年轻的SCC患者与老年患者相比,与尼古丁酸有积极的相关性. 在年轻的SCC女性中,与RANTES,miR-20b-5p和miR-155-5p相关的代谢物升高.
结论:
- 更年期状态显著影响宫微生物组合及其代谢相互作用,影响宫瘤的进展.
- 宫微环境,包括微生物和代谢特征的特定年龄差异是显而易见的.
- 这些发现支持针对不同年龄组和更年期阶段的宫癌开发个性化医疗方法.
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