在发酵的卜汁中发现的酵母菌株的隔离,鉴定和体外益生菌特性
Ilkin Sengun1, Husniye Tansel Yalcin2, Yigit Terzi3
1Engineering Faculty, Food Engineering Department, Ege University, Izmir, Bornova 35100 Türkiye.
Journal of food science and technology
|July 31, 2025
概括
来自土耳其发酵卜汁的酵母菌株显示出有前途的益生菌潜力. 这些酵母表现出对酸性条件,胆盐的耐受性,并表现出抗菌性质,这表明它们在新型食品中使用.
科学领域:
- 食品微生物学 食品微生物学
- 益生菌研究 益生菌研究
- 酵母发酵 酵母发酵的方法
背景情况:
- 发酵食品是有益微生物的来源.
- 传统的发酵产品含有潜在益生菌性质的酵母.
- 卜汁发酵是土耳其的传统做法.
研究的目的:
- 为了研究从发酵的卜汁中分离出来的酵母的益生菌特性.
- 确定在功能性食品中具有潜在应用的酵母菌种.
- 评估这些酵母菌株的耐受性和功能性质.
主要方法:
- 从发酵的卜汁中分离和识别酵母菌株.
- 在各种pH值和胆盐条件下对酵母活性的评估.
- 对胃肠道酶,NaCl的耐受性和抗菌活性的评估.
- 对细胞表面的疏水性,自聚和同聚与沙门氏菌Typhimurium的分析.
主要成果:
- 确定了主要的酵母物种:Pichia kudriavzevii,P. fermentans,P. occidentalis和P. manshurica. 这些都是主要的酵母物种.
- 所有测试的菌株在低pH (2-4) 条件下都表现出活力,对胆汁盐,,素和胰腺素有耐受性.
- 观察到显著的细胞表面疏水性和自身聚合.
- 与沙门氏菌Typhimurium的联合聚合在菌株之间有所不同,这表明潜在的病原体抑制.
结论:
- 从发酵的卜汁中分离出来的酵母菌株具有显著的益生菌潜力.
- 这些酵母对恶劣的胃肠道条件和功能性质具有耐受性.
- 已识别的酵母菌株可以被开发成新的益生菌食品或补充剂.
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