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Updated: Sep 13, 2025

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

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在和蛋白质中可见光诱导的二硫化键减少

Keting Zhou1, Xiaoyue Yang1, Hanlin Ren1

  • 1MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.

The journal of physical chemistry. A
|July 31, 2025
PubMed
概括

一个新的可见光系统有效地减少了用于质谱 (MS) 分析的蛋白质中的二硫化物键. 这种方法使和蛋白质测序成为可能,为生物制药研究提供了基于UV的技术的温和替代方案.

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