相关实验视频
Updated: Sep 13, 2025

Probiotic Studies in Neonatal Mice Using Gavage
Published on: January 27, 2019
肠道微生物群的发展,抗生素耐药性,以及早期婴儿期相关的围产期因素
Pianpian Fan1, Jinqian Ma1,2, Qianwen Shen3
1Ministry of Education and Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
婴儿发育中的肠道微生物组在最初的六个月内显示出越来越多的多样性和抗生素耐药性基因. 分娩方式和辅助生殖技术 (ART) 影响这些早期变化.
科学领域:
- 微生物学 微生物学
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 早期的肠道微生物群对婴儿健康和免疫系统发育至关重要.
- 婴儿的抗生素耐药性受各种围产期因素的影响.
- 了解婴儿肠道微生物群的纵向变化对于识别健康风险至关重要.
研究的目的:
- 为了研究肠道微生物群和抗生素耐药性的纵向变化,在生命的前六个月.
- 检查辅助生殖技术 (ART),分娩方式和婴儿性别对早期肠道微生物群发育的影响.
- 在婴儿肠道微生物组中识别主导抗生素耐药性基因.
主要方法:
- 155名中国婴儿出生时,42天,3个月和6个月的便样本的基因组测序.
- 对α多样性指数 (ACE,Chao,Sobs,Shannon,Pielou's evenness) 和微生物组成的分析.
- 对抗生素耐药性基因的识别和量化.
主要成果:
- 肠道微生物群的多样性和抗生素耐药性基因的丰富性随着年龄的增长而增加,在阴道出生的婴儿中.
- 剖腹产与较慢的α多样性增加和较低的Bacteroidetes和Bacteroides丰度有关.
- 与非ART婴儿 (阴道分娩) 相比,通过ART怀孕的婴儿在42天内显示出略高的α多样性.
- 确定的主要抗生素耐药性基因是macB和msbA.
结论:
- 婴儿肠道微生物群的组成和多样性在最初的六个月内显著发展.
- 产生的方式和ART概念与婴儿早期肠道微生物群的改变有关.
- 这些发现凸显了围产期因素在塑造婴儿肠道微生物组和抗药组中的关键作用.
更多相关视频
08:33Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
Published on: November 17, 2018
08:50A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
Published on: June 24, 2020
相关概念视频
Anatomy of the Intestines
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Development of Antibiotic Resistance
Microorganisms in Medicine and Therapeutics
Development of Immunocompetence
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Factors Affecting the Risk of Infection
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...