自然Nrf2激活剂调节白血病K-562细胞中的抗氧化基因表达和亡
Iason-Spyridon Patergiannakis1, Sofia K Georgiou-Siafis2, Lefkothea C Papadopoulou2
1Laboratory of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Thessaly, Trikalon 224, 43100, Karditsa, Thessaly, Greece.
Medical oncology (Northwood, London, England)
|July 31, 2025
概括
这项研究探讨了22种天然产品提取物对它们对白血病细胞的影响. 一些提取物,包括Thymus vulgaris,表现出显著的抗氧化,细胞毒性和Nrf2通路调节活动,突出显示了它们的治疗潜力.
科学领域:
- 药理学 药理学是指药理学的学科.
- 自然产品研究 自然产品研究
- 癌症生物学 癌症生物学
背景情况:
- 自然产品 (NPs) 在传统医学和癌症研究中至关重要.
- 它们的治疗效果往往涉及氧化还原敏感通道,特别是核因素红色素2相关因子2 (Nrf2) 途径.
- Nrf2调节抗氧化反应和细胞防御机制,这对癌症化学预防至关重要.
研究的目的:
- 为了评估22种民族医学天然产品提取物的抗氧化能力,细胞毒性和亲亡活性.
- 评估这些提取物在人类白血病K-562细胞模型中的Nrf2调节作用.
- 确定具有显著化学预防潜力的特定天然产品.
主要方法:
- 总含量 (TPC) 和激素清除活性 (DPPH,ABTS试验) 的量化.
- 使用AlamarBlue测定和通过caspase-3活性诱导亡的细胞毒性影响的评估.
- 使用RT-qPCR对Nrf2目标基因表达 (HO-1,NQO1,GCLC) 的分析.
主要成果:
- 提取物表现出多种抗氧化和细胞毒性活动;花粉杆菌,花粉和杆菌表现出高的TPC和抗氧化潜力.
- 罗迪奥拉花表现出最强的细胞毒性作用,而Hypericum perforatum则诱导了最高的caspase-3活性.
- 乳腺,罗迪奥拉和其他物种显著上调了Nrf2基因,而T. vulgaris表现出最大的诱导作用.
结论:
- 传统上使用的天然产品具有显著的药理潜力,可作为氧化还原信号的调节剂和白血病细胞中亡的诱导剂.
- 鉴定的提取物,特别是激活Nrf2通路的提取物,需要进一步研究癌症化学预防和治疗.
- 这项研究验证了精选的NP的民族医学用途,并为其开发提供了科学基础.
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