优化ISO标准的微生物学技术,从家禽样本中分离Campylobacter,同时应对产生大肠杆菌的扩展光谱β-乳糖酶的挑战
Keya Ghosh1, Tahia Ahmed Logno1, Tridip Das2,3
1Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University, Chittagong, Bangladesh.
PloS one
|July 31, 2025
概括
这项研究确定了产生大肠杆菌的扩展谱β-乳糖酶 (ESBL) 作为阻碍坎皮洛巴克特分离的污染物. 用tazobactam补充选择性介质有效抑制了大肠杆菌,改善了LMIC环境中的坎比洛巴克特检测.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 兽医公共卫生 兽医公共卫生
背景情况:
- 在低收入和中等收入国家 (LMICs) 中,标准化Campylobacter隔离协议 (ISO 10272:2017) 面临着挑战.
- 产生大肠杆菌的扩展谱β-乳糖酶 (ESBL) 的过度生长阻碍了在LMIC中从肉样中分离Campylobacter.
研究的目的:
- 确定在隔离Campylobacter期间导致过度生长的污染物.
- 确定减轻ESBL产生大肠杆菌过度生长的方法.
- 为LMIC设置优化Campylobacter隔离协议.
主要方法:
- 选了25个非坎皮洛巴克特菌分离物从的样中进行物种识别和ESBL基因的选.
- 对于产生ESBL的大肠杆菌,确定了tazobactam的最低抑制度 (MIC).
- 修改了ISO 10272:2017协议,通过补充选择性丰富 (普雷斯顿) 和 (mCCDA) 添加tazobactam.
主要成果:
- 所有确定的污染物都是产生ESBL的大肠杆菌,携带blaTEM,blaCTX或blaSHV基因.
- 塔佐巴克坦在128 mg/L的剂量抑制了在普雷斯顿中产生ESBL的大肠杆菌,而不会影响坎比洛巴克特物种.
- 用4mg/L的tazobactam补充mCCDA抑制了产生ESBL的大肠杆菌,提高了Campylobacter分离的效率.
结论:
- 在mCCDA上直接注射肉肉的材料,并补充4mg/L的tazobactam,是对坎皮洛巴克特监测的一种经济有效的修改.
- 这种优化的方法通过防止大肠杆菌过度生长,改善了坎比洛巴克的检测,特别是在LMIC环境中.
- 修改后的协议提供了一个实用的替代标准ISO 10272:2017的Campylobacter隔离从 cecal样本.
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