相关实验视频

Updated: Sep 13, 2025

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

15.1K

SCoTCH-seq显示,5-基甲基细胞因在DNA链中编码调控信息

Jack S Hardwick1, Somdutta Dhir2, Angie Kirchner2

  • 1Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|July 31, 2025
PubMed
概括

新的SCoTCH-seq技术对DNA进行解码.

关键词:
5 - 基甲基细胞氨基-5-基甲基细胞氨基-5-基甲基细胞氨基5甲基细胞氨酸5甲基细胞氨酸在DNA测序过程中,DNA测序表观遗传学是指表观遗传学.

更多相关视频

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA06:26

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Published on: October 5, 2012

12.1K
Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry07:13

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Published on: August 16, 2016

7.4K

相关概念视频

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
10.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.6K
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.5K
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.5K
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K