优化Sihuang化合物与细菌菌素 (Bacillus subtilis) 的发酵过程及其对大肠杆菌感染的疗效以及 brojlers的生长性能
Lin Liao1, Weihua Zhang1, Yuheng Liu2
1College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Poultry science
|July 31, 2025
概括
益生菌发酵将传统的西黄 (SH) 草药配方增强为发酵的SH (FSH),改善其对 brojlers中的禽病原性大肠杆菌 (APEC) 的治疗作用. FSH增强免疫力,肠道健康和生长性能,提供了一个有前途的抗生素替代品.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
- 动物营养 动物营养
- 草药是一种草药.
背景情况:
- 西黄 (SH) 是一种传统的草药配方,用于治疗大肠杆菌病,但其抗菌活性有限.
- 提高SH的有效性和生物活动对于改善家禽健康和产量至关重要.
- 益生菌发酵提供了一种新的方法来修改草药化合物并提高其治疗潜力.
研究的目的:
- 开发和优化一个体外益生菌发酵的Sihuang (FSH) 配方.
- 研究FSH在受禽病原性大肠杆菌 (APEC) 挑战的肉中的生理特征和治疗疗效.
- 为了在体外和体内比较FSH与未发酵SH的影响.
主要方法:
- 使用响应表面方法来优化Bacillus subtilis的发酵条件.
- 实验室和体内研究在APEC挑战型肉上进行,比较FSH和SH.
- 评估了生理参数,抗氧化能力,免疫功能,肠道健康标志物和生长性能.
主要成果:
- 细菌细菌发酵显著增加了SH中总的黄酸盐,贝卡林和柏柏林含量,同时降低了pH值.
- FSH显著增强了总抗氧化能力 (T-AOC),免疫功能以及谷氨过氧化酶 (GPx) 和催化酶 (CAT) 的活性.
- 与SH相比,FSH通过增加乳酸菌的丰富性,α-氨基酶活性和紧结蛋白 (TJP) 基因表达来改善肠道健康,从而导致更好的生长性能 (ADG,FCR).
结论:
- 使用Bacillus subtilis进行益生菌发酵有效地提高了Sihuang (SH) 的生物活性化合物含量和治疗效果.
- 发酵西黄 (FSH) 在改善抗氧化剂状态,免疫功能,肠道完整性和APEC挑战型肉生长方面表现出优异的体内表现.
- 在家禽业中,FSH是一种有前途的抗生素替代品,与传统的SH相比,在降低剂量的情况下提供更高的疗效.
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