使用气体染色学和质谱技术,同时确定人类血液 (血清/血) 中的持久性和非持久性有机污染物的方法方法
Carolina M Bustamante1, Paula Ruiz2, Atiya B Rifat3
1Institute of Environmental Assessment and Water Research (IDAEA-CSIC). Barcelona, Catalonia, Spain; Faculty of Chemistry. Universitat de Barcelona (UB). Barcelona, Catalonia, Spain.
一种新方法有效地测量了人类血清中的47种持久性有机污染物 (POP) 和其他化学物质. 这种经过验证的技术具有很高的灵敏度和准确性,支持对健康风险评估和监管评估的关键生物监测.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 人类生物监测对于评估暴露在持久性有机污染物 (POP) 中至关重要.
- 追踪POPs有助于评估健康风险和监管行动的影响.
- 准确的分析方法对于可靠的生物监测数据至关重要.
研究的目的:
- 开发和验证一种优化的分析方法来确定人类血清中广泛的POPs.
- 建立一种灵敏,精确和准确的方法,适用于常规的人类生物监测.
- 在分析中包括持久性和选择非持久性化学物质.
主要方法:
- 一次液体液体提取500μL血清/血.
- 气相色谱与电子捕获检测 (GC-ECD) 对于有机.
- 气相色谱与负化学电离模式 (GC-MS-NCI) 中的质谱相结合,用于聚二乙烯 (PBDE).
主要成果:
- 该方法量化了47种化合物,包括各种POP和三个不持久化学物质.
- 高灵敏度通过低检测极限 (0.0016-0.012 ng/mL) 和定量 (0.0029-0.021 ng/mL) 实现.
- 验证了线性 (R2 > 0.9962),精度 (CV <25%) 和准确性 (41/47化合物的回收率为70-130%),具有最小的矩阵效应.
结论:
- 开发的方法是高效的,实用的,适合常规的人类生物监测.
- 它为广泛的环境污染物提供可靠和敏感的测量.
- 通过成功参加能力测试 (AMAP环测试) 确认方法可靠性.
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