对咖啡内生菌酵母进行评估,以有效地去除粘液,持续提高咖啡生产质量
Yukai Suo1, Na Yang1, Hongmin Yang2
1Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes, State Ethnic Affairs Commission, Yunnan Minzu University, Kunming 650504, China; Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming 650031, China.
Food chemistry
|July 31, 2025
概括
Pichia kluyveri YE7有效地去除咖啡桃粘液,即使在寒冷的条件下. 这种酵母发酵在烤过程中对豆类化合物的影响最小,为工业应用保留了感官咖啡质量.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 咖啡植物中的内菌酵母已知,但它们降解粘液的潜力在很大程度上未被研究.
- 高效的粘液去除对于湿咖啡加工至关重要,影响了咖啡豆的质量.
研究的目的:
- 从咖啡桃中分离和特征化解酵母,以去除粘液.
- 评估最佳酵母菌株Pichia kluyveri YE7在咖啡发酵中的表现及其对豆子成分和感官属性的影响.
主要方法:
- 从咖啡桃中分离和识别了42种解酵母菌株.
- 评估选定的菌株的粘液降解效率,耐寒性和酶潜力 (pectinases,cellulases,hemicellulases).
- 使用P. kluyveri YE7进行发酵后对绿色和烤咖啡豆的化学变化 () 进行分析.
- 使用电子舌头分析评估发酵和未发酵豆的感官属性.
主要成果:
- Pichia kluyveri YE7表现出优异的粘液去除 (4-12h在5-30°C) 并拥有相关水解酶的基因.
- 发酵改变了绿豆中的基含量,但在烤后降低了,对最终的挥发性概况的影响最小.
- 电子舌头分析显示,发酵和未发酵豆在烤后的感觉属性相似.
结论:
- 由于其高效的粘液降解和耐寒性,Pichia kluyveri YE7对于大规模的湿咖啡发酵非常有效.
- 这种酵母在最终的烤咖啡中提供了稳定的感觉特征,使其成为工业应用的有希望的候选人.
- 该研究支持使用P. kluyveri YE7在不同的环境条件下进行高效和质量维护的咖啡加工.
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