集群占用和氧化状态依赖于Yersinia enterocolitica IscR的DNA结合
Elizabeth Gray1, Miaomiao Gao1, Justin M Bradley1
1Centre for Molecular and Structural Biochemistry, School of Chemistry, Pharmacy and Pharmacology, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.
Journal of inorganic biochemistry
|July 31, 2025
概括
铁硫 (Fe-S) 集群是重要的蛋白质辅因子. 对Yersinia enterocolitica IscR的研究表明,其Fe-S集群氧化状态影响DNA结合,这对于调节必要的细胞机械至关重要.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 铁硫 (Fe-S) 集群是许多细胞过程中必不可少的蛋白质辅因子.
- 铁硫集群 (ISC) 系统汇集了这些共因子,其调节涉及转录调节器IscR.
- 触发IscR监管功能的精确信号尚不清楚.
研究的目的:
- 研究来自Yersinia enterocolitica (YeIscR) 的IscR同类及其功能特征.
- 为了确定Fe-S集群的氧化状态在YeIscR的DNA结合活性中的作用.
- 将YeIscR功能与其大肠杆菌对应物进行比较.
主要方法:
- 对YeIscR及其 [2Fe-2S] 集群的净化和表征.
- 使用氧气和其他氧化剂的氧化研究.
- 使用铁化剂评估集群可变性.
- 用DNA结合测试来评估集群氧化状态和损失的影响.
主要成果:
- YeIscR结合了一个 [2Fe-2S] 集群,可能由三个半氨酸和一个氨酸协调.
- 暴露于氧化剂迅速氧化了集群从+1到+2状态,随后是缓慢的集群损失.
- 对于铁化剂,YeIscR集群相对稳定.
- 集群氧化显著降低了DNA结合亲和力 (在集群损失时大约减少了10倍).
结论:
- 在YeIscR中的Fe-S集群的氧化状态对于其DNA结合活性至关重要.
- YeIscR集群的氧化导致DNA结合减少,这表明一个调节机制.
- 这些发现提供了对IscR同类在调节Fe-S集群稳态中的保存功能的见解.
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