vPro-MS可以通过非向的蛋白质组学从患者样本中识别人类致病病毒
Marica Grossegesse1, Fabian Horn2, Andreas Kurth3
1Robert Koch Institute, Centre for Biological Threats and Special Pathogens: Highly Pathogenic Viruses (ZBS 1), WHO Collaboration Center for Emerging Threats and Special Pathogens, Berlin, Germany.
Nature communications
|July 31, 2025
概括
这项研究引入了病毒蛋白质组学 (vPro-MS) 用于在患者样本中进行非向病毒识别. 这种方法具有很高的特异性和灵敏性,补充PCR和转基因组学,用于更广泛的病毒诊断.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 病毒学 病毒学
- 生物信息学是一种生物信息学.
背景情况:
- 目前的病毒诊断依赖于针对性的方法,如PCR和免疫测试.
- 转基因组学提供非向的病毒基因组识别,但可能是复杂的.
- 对于更广泛的病毒检测,需要使用补充的非向方法.
研究的目的:
- 引入蛋白质组学作为一种新的,非向的方法来识别人类致病病毒.
- 开发和验证用于临床样本分析的病毒蛋白质学工作流 (vPro-MS).
- 在特异性,灵敏性和吞吐量方面评估vPro-MS的性能.
主要方法:
- 开发一种病毒蛋白质组学工作流程 (vPro-MS),使用来自UniProtKB.B的in-silico库.
- 创建一个评分算法 (vProID评分) 来从蛋白质组学数据中确定病毒.
- 应用基于diaPASEF的数据采集,用于高通量分析 (高达60个样本/天).
主要成果:
- 在221个不同的临床样本 (血,拭子,细胞培养) 中,vPro-MS表现出高特异性 (>99.9%),检测出17种病毒.
- 鼻抽样中对SARS-CoV-2检测的敏感性与PCR (Ct27) 相似,定量准确性与元基因组学相似.
- 该工作流允许集成到大规模的蛋白质组学研究中,以发现新型病毒感染.
结论:
- 病毒蛋白质组学 (vPro-MS) 是一种可行的非向方法,用于识别人类致病病毒.
- vPro-MS提供高特异性和灵敏度,补充现有的诊断技术.
- 这种方法有助于在生物流体的大规模蛋白质组学研究中发现未知的病毒感染.
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