Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.9K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

252
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
252
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

High-efficiency TadA cytosine base editors for precise modelling of human disease variants.

Nature biomedical engineering·2026
Same author

Expansion of Molecular and Clinical Aspects of EPS8L2 (DFNB106)-Associated Hearing Loss Emphasizes a Potential Therapeutic Window.

Molecular neurobiology·2026
Same author

Complete <i>de novo</i> assembly and re-annotation of the zebrafish genome.

bioRxiv : the preprint server for biology·2025
Same author

The chromosome-level genome assembly of the slug Deroceras laeve facilitates its use as a comparative model of regeneration.

G3 (Bethesda, Md.)·2025
Same author

Rationally Designed TadA-Derived Cytosine Editors Enable Context-Independent Zebrafish Genome Editing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

The transcription factors Tfeb and Tfe3 are required for survival and embryonic development of pancreas and liver in zebrafish.

PLoS genetics·2025

相关实验视频

Updated: Sep 13, 2025

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.3K

在脊椎动物模型中使用基于CRISPR的功能基因组学工具.

Gaurav K Varshney1, Shawn M Burgess2

  • 1Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. Gaurav-varshney@omrf.org.

Experimental & molecular medicine
|July 31, 2025
PubMed
概括
此摘要是机器生成的。

在脊椎动物模型中,CRISPR-Cas技术彻底改变了功能基因组学. 这些先进的基因编辑工具,包括基因和主要编辑器,加速了基因功能发现和潜在治疗方法的疾病建模.

更多相关视频

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
05:34

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Published on: February 1, 2018

8.5K
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.2K

相关实验视频

Last Updated: Sep 13, 2025

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.3K
Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
05:34

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Published on: February 1, 2018

8.5K
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.2K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 克里斯普尔-卡斯技术已经改变了研究中的基因操纵.
  • 在脊椎动物模型中,如小鼠和斑马鱼,CRISPR的应用非常普遍.
  • 进步包括高通量选,敲进等位基因和大规模选.

研究的目的:

  • 审查基于CRISPR的工具在脊椎动物模型中的影响.
  • 突出CRISPR在功能基因组学和疾病建模中的实用性.
  • 讨论创新的CRISPR技术及其应用.

主要方法:

  • 适应CRISPR-Cas用于高通量突变发生.
  • 开发用于单核酸修改的基数编辑器.
  • 实现主要编辑器,以便在没有双链断裂的情况下精确编辑.
  • 利用CRISPR干扰和激活进行转录调制.
  • 采用像MIC-Drop和Perturb-seq这样的新方法进行体内查.

主要成果:

  • 在脊椎动物模型中,CRISPR工具可以进行精确的基因操纵.
  • 克里斯普尔促进了对基因功能在发育,生理学和病理学的理解.
  • 创新的CRISPR技术扩大了遗传研究的工具包.
  • 基于CRISPR的基因疗法在临床转化方面表现有前途,包括状细胞疾病.

结论:

  • 基于CRISPR的工具对脊椎动物的功能基因组学产生了变革性的影响.
  • 这些技术对于推进疾病建模和理解复杂的生物过程至关重要.
  • 正在进行的CRISPR方法的开发有望在研究和治疗方面取得进一步的突破.