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相关概念视频

Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
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Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

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Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
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The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

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Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
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TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

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The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
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Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

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De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
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相关实验视频

Updated: Sep 13, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
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LMOD2与ACTC1的相互作用调节了肌原分化.

Kaiming Wang1, Caihong Liu1, Lei Yi1

  • 1College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China.

BMC genomics
|July 31, 2025
PubMed
概括

雷奥莫丁2 (LMOD2) 淘汰抑制肌细胞增殖并改变肌肉纤维类型,影响骨肌肉发育. MyoG通过转录调节LMOD2,而miR-335-3p负面控制其表达,揭示了新的分子标.

关键词:
在ACTC1中,在LMOD2中使用LMOD2.这是一次Knockout.脑细胞质细胞 脑细胞质细胞是什么?骨架肌肉是一个骨肌肉.

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Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
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Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

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相关实验视频

Last Updated: Sep 13, 2025

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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
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Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
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科学领域:

  • 肌肉生理学和分子生物学.
  • 骨肌肉中的基因表达和调节.
  • 肌肉发育和再生的细胞机制.

背景情况:

  • 骨肌肉是最大的哺乳动物组织,对新陈代谢和平衡至关重要.
  • 肌肉发育和再生涉及复杂的基因表达调节.
  • 莱奥莫丁2 (LMOD2) 在心脏和骨肌中表达,但其在骨肌发育中的功能尚不清楚.

研究的目的:

  • 研究莱奥莫丁2 (LMOD2) 在骨肌肉发育中的生理功能和调节机制.
  • 确定LMOD2.2的上游调节器和相互作用蛋白.
  • 探索LMOD2在肌细胞增殖,分化和肌肉纤维类型决定中的作用.

主要方法:

  • 在猪组织和C2C12细胞中检查了LMOD2表达.
  • 在C2C12细胞中执行LMOD2淘汰,以评估功能影响.
  • 使用转录组分析 (RNA-seq) 和共免疫沉 (Co-IP) 试验.
  • 在实体研究中,使用晶状病毒介导的LMOD2倒置进行了实验.

主要成果:

  • 随着肌肉生长,LMOD2表达量下降,但在受伤后增加,并且随着C2C12细胞增殖/分化而增加.
  • LMOD2淘汰改变了肌肉纤维类型 (抑制了MyHC-I/2b,促进了MyHC-2a/2x),抑制了细胞活力,并减少了PAX7的表达.
  • MyoG被确定为一个LMOD2转录因子,miR-335-3p作为负调节剂,ACTC1作为相互作用蛋白.
  • 在体内,LMOD2敲击降低了肌肉质量,并抑制了特定的MyHC异型.

结论:

  • LMOD2淘汰赛抑制了肌细胞增殖,并改变了骨肌肉纤维类型的组成.
  • MyoG和miR-335-3p是LMOD2表达的关键调节者.
  • LMOD2与ACTC1相互作用以调节肌原分化.
  • LMOD2代表了骨肌肉发育的潜在治疗标.