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在Fagopyrum物种中由马射线诱导的跨代遗传和表观遗传变化
Katarzyna Sala-Cholewa1, Magdalena Rojek-Jelonek1, Jolanta Kwasniewska2
1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, Katowice, 40-032, Poland.
玛射线在麦物种中诱导跨代DNA甲基化变化. 虽然第一代表现出不稳定性,但第三代表现出恢复,具有重要的物种特异性表观遗传变化,与植物育种相关.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 植物科学 植物科学
- 遗传学 是一个遗传学.
背景情况:
- 压力诱导的DNA变化可能是遗传的,影响植物的改善.
- 基因甲基化是一种表观遗传机制,驱动着遗传变异.
- 麦种 (Fagopyrum esculentum和F. tataricum) 是重要的食品和药用作物.
研究的目的:
- 研究两个Fagopyrum物种的跨代基因组和表观基因组变化.
- 分析马射线治疗对跨代DNA甲基化模式的影响.
主要方法:
- 对Fagopyrum物种的第1代和第3代进行分析.
- 玛射线治疗的应用.
- 评估基因组不稳定性 (DNA碎片化,微核,细胞周期).
- 在根组织中评估DNA甲基化水平和模式.
主要成果:
- 在第一代观察到的基因组不稳定性,在第三代恢复.
- 马射线压力改变了DNA甲基化模式,无论是物种还是世代.
- 观察到对玛射线的特定物种反应,F. esculentum在第1代中显示出更高的甲基化增加.
- 第三代DNA甲基化变化仅在F. esculentum中显著.
结论:
- 马射线对麦的DNA甲基化产生影响,其影响取决于物种.
- 对照和第三代之间的DNA甲基化显著差异仅在F. esculentum中发现.
- 马射线诱导的突变产生了一个稳定的,潜在的自我授粉的F. tataricum突变.
- 了解转基因治疗诱导的DNA甲基化变化对于育种计划至关重要.
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