关于使用基于酸的预处理协议进行牙乳液碳和氧分析的方法考虑
Karolina Varkulevičiūtė1,2, Christine Winter-Schuh1,2, Cheryl A Makarewicz1,2
1Institute of Prehistoric and Protohistoric Archaeology, University of Kiel, Kiel, Germany.
Rapid communications in mass spectrometry : RCM
|August 1, 2025
概括
化学预处理会改变牙面膜的同位素值. 研究人员建议避免过氧化 (H2O2) 和低化 (NaClO),并使用短时间的酸处理来保存原始的碳 (δ13C) 和氧 (δ18O) 同位素信号.
科学领域:
- 考古学测量是指考古学测量的方法.
- 古生态环境重建的重建
- 生物地质化学生物地质化学
背景情况:
- 化学预处理对于消除考古牙面膜的基因变化至关重要.
- 使用氧化试剂 (NaClO,H2O2) 和弱酸 (CH3COOH) 的常用方法可以改变质的化学成分和稳定同位素值 (δ13C,δ18O).
研究的目的:
- 重新检查常见预处理方案对生物酸盐 δ13C 和 δ18O 值的影响.
- 用ATR-FTIR光谱学研究基因变化与测量同位素值之间的关系.
主要方法:
- 十个常见的预处理协议涉及NaClO,H2O2和/或CH3COOH被应用于现代和考古牙面膜粉.
- 减弱总反射率-里埃变换红外光谱学 (ATR-FTIR) 评估了处理前后的保存和对基因改变.
- 在处理前和处理后测量了δ13C和δ18O值,以检测同位素变化.
主要成果:
- 所有测试的预处理方案都在两种样本类型中诱导了δ13C和δ18O值的变化 (高达±1.5‰).
- 大多数经过处理的样本显示结晶度增加,表明再结晶.
- 这些变化表明污染物和基因变化的去除,以及质碳酸盐的溶解和重组,改变了体内同位素信号.
结论:
- 过氧化 (H2O2) 和低化 (NaClO) 的治疗是不推的,因为质结构和同位素比的不必要变化.
- 建议进行短期的酸处理,以避免长时间暴露在酸中,然后再结晶,保持体内同位素值.
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