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酸染料的蛋白封装用于高性能NIR-II成像指导光动力疗法

Lang Bai^1,2, Yiyang Jia^3,4, Zihan Wang⁵

¹State Key Laboratory of Supramolecular Structure and Materials, Center for Supramolecular Chemical Biology, College of Chemistry, Jilin University, Changchun 130012, P.R. China.

Chemical & biomedical imaging

|August 1, 2025

概括

研究人员使用DTT开发了一种新的白蛋白封装策略,以改善光动力疗法 (PDT) 剂. 这种方法增强了瘤积累和PDT在癌症治疗中的有效性.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
癌症治疗癌症治疗

背景情况:

专封装是一种有前途的药物输送策略,特别是在光动力疗法 (PDT) 药物中.
含有化物的近红外 (NIR) 色剂是有效的PDT剂,但由于尺寸小 (<10 nm),与白蛋白复合时显示出有限的瘤积累,从而损害了它们的有效性.
现有的方法难以平衡PDT的效率,成像能力和瘤向.

研究的目的:

开发一种改进的胺封装策略,用于含化物NIR氨酸染料,以提高其光动力学疗法 (PDT) 的疗效.
优化这些药物载入纳米颗粒在瘤部位的积累,以改善癌症治疗.
维持或增强近红外 (NIR) 亮度用于成像引导的PDT.

主要方法:

使用DTT促进的封装策略来修改白蛋白结构.
专蛋白内的二硫化物键被破坏,改变了纳米粒子的大小和性质.
修改的白蛋白染料复合物 (IR-6B3@DTT-HSA) 的特点是尺寸,单片氧释放,亮度和光稳定性.
进行了体内实验,以评估瘤积累和治疗疗效.

主要成果:

DTT处理松动了白蛋白外,增加了纳米粒子大小,同时保持了单片氧释放,部分NIR亮度和光稳定性.
开发的IR-6B3@DTT-HSA纳米颗粒在体内显示出瘤的快速积累.
增强的积累促进了灵活的治疗时间,提高了PDT的疗效.
该策略成功地解决了较小的白蛋白染料复合物的有限瘤积累问题.

结论:

通过DTT促进的白蛋白封装是一种有效的策略,可以增强NIR氨酸染料基PDT剂的瘤向和治疗疗效.
这种方法改善了纳米粒子在瘤中的积累,使得癌症治疗更有效.
开发的方法有可能在成像导向的癌症治疗中得到更广泛的应用.

关键词:

在NIR-II成像系统中使用.专辑蛋白 (albumin) 是一种酸染料的使用方法纳米颗粒是一种纳米粒子.光动力学疗法是一种光动力学疗法.

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