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甲状腺刺激激素受体融合蛋白的表达,免疫性和临床意义分析
1Department of Endocrinology and Metabolism, The Second Hospital and Clinical Medical School, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
使用新型融合蛋白来研究甲状腺刺激激素受体 (TSHR) 抗体. 这些蛋白质在甲状腺功能过高和甲状腺功能低下之间进行了区分,有助于自身免疫甲状腺疾病的研究和诊断开发.
科学领域:
- 内分泌学和免疫学.
- 分子生物学分子生物学
- 自免疫性疾病 自免疫性疾病
背景情况:
- 甲状腺功能由TSHR调节,与抗体 (TRAb) 引起甲状腺功能障碍.
- 了解TSHR结构域对于诊断和治疗自身免疫性甲状腺疾病至关重要.
研究的目的:
- 调查TSHR融合蛋白的免疫性和临床意义.
- 为TRAb检测开发新的诊断工具.
主要方法:
- 创建并合成了人类TSHR (hTSHR) 融合蛋白碎片 (hTSHR289,hTSHR290,hTSHR410).
- 使用ELISA进行体外结合试验,对患者血清进行测试.
- 在小鼠身上进行了体内研究,包括自身抗体测量,甲状腺功能评估,组织学和流细胞计.
主要成果:
- hTSHR290显示了与甲状腺功能低下患者的TRAb+血清的高结合.
- hTSHR289在甲状腺功能升高患者血清中的特定结合刺激抗体.
- 在体内研究表明,hTSHR289组的甲状腺功能过高的病理和hTSHR290组的甲状腺功能低下的病理.
- 融合蛋白诱导了明显的T细胞亚群变化.
结论:
- TSHR融合蛋白具有差异性免疫性和临床相关性.
- hTSHR289和hTSHR290是开发用于TRAb检测的ELISA套件的有希望的候选人.
- 这些发现为了解自身免疫性甲状腺疾病机制和开发新诊断方法提供了基础.
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