单聚糖可溶性微针用于无痛局部麻醉:制造,表征和体外神经成像
João M S P Leite1, Ana C Q Silva1, Ana Jesus2,3
1CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro 3810-193, Portugal.
ACS biomaterials science & engineering
|August 1, 2025
概括
这项研究开发了可溶性Pullulan微针,用于快速,微创的利多卡因输送. 微针有效地通过皮肤输送麻醉剂,与传统方法相比,显示出高安全性和效率.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 皮肤病学 皮肤病学
背景情况:
- 微针 (MNs) 提供了一种对传统药物管理方法的最小侵入性替代方案.
- 基于多糖的MNs提供了一个有前途的生物降解平台,用于通过皮肤递送药物.
- 利多卡因是一种广泛使用的局部麻醉剂,需要有效和快速的输送方法.
研究的目的:
- 开发和表征单组件普鲁兰微针用于通过皮肤输送利多卡因.
- 评估Pullulan MNs的机械强度,皮肤透和溶解特性.
- 评估基于Pullulan MN的利多卡因输送系统的安全性和有效性.
主要方法:
- 微型模具被用来制造Pullulan微针.
- 机械测试评估了针的强度.
- 使用切除的人体皮肤和皮肤模型来评估角层透和溶解.
- 在HaCaT,3T3和RAW 264.7细胞系上使用resazurin代谢试验测试了细胞相容性.
- 时间延迟光显微镜比较利多卡因释放的疗效.
主要成果:
- 普鲁兰微针显示出高的机械强度 (高达1.5N/针).
- 在10分钟内成功透切除的人类腹部皮肤,并在皮肤模型中溶解.
- 10分钟后,在贴片中的利多卡因保留率为26% (糖凝) 和32% (猪皮).
- 超过80%的细胞活力证实了多个细胞系的细胞相容性.
- 经MN递送的利多卡因在感觉神经元上表现出与纯溶液相当的活性.
结论:
- 可溶性Pullulan微针是一种安全有效的通过皮肤输送利多卡因的系统.
- 这种系统提供了快速起作用 (<10分钟) 和高效的药物释放.
- 普鲁兰MNs代表了传统局部麻醉剂施用方法的有希望的替代方案.
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