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异马尔多利高糖的生产使用来自Zalaria sp.的α-葡萄糖酶活性. 他3,一个产生的果脂糖糖的酵母酵母

Haruki Matsuya¹, Mayumi Maeda², Kenji Maehashi^1,2

¹Department of Fermentation Science and Technology, Graduate School of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-Ku, Tokyo, 156-8502, Japan.

Current microbiology

|August 1, 2025

概括

这项研究表明Zalaria sp. 希姆3酵母可以使用它的α-葡萄糖酶 (AGase) 生产异型多酸糖 (IMO). 这种酵母是一种有前途的工业来源,可以生产多种寡糖类,如IMO和果寡糖类 (FOS).

科学领域:

生物技术是生物技术.
酶学是一种酶学.
微生物学微生物学

背景情况:

酵母菌株正在探索碳水化合物生产中的新型工业应用.
扎拉里亚 sp. 扎拉里亚 sp. 之前以生产果类糖 (FOS) 而闻名的Him3目前正在研究其它类糖的功能.
了解酵母碳源利用率对于优化酶生产至关重要.

研究的目的:

发展异多糖 (IMO) 生产作为Zalaria sp.的新型工业应用. 他3. 他3. 他3.
描述来自Zalaria sp.的α-葡萄糖酶 (AGase) 酶的特征. 在IMO合成中使用Him3.
为了评估Zalaria sp.的潜力. Him3作为一种酶资源,用于多重寡糖类的生产.

主要方法:

Zalaria sp. 的碳源利用情况使用API 50CH.Him3进行了评估.
这种菌株在马尔托斯基质中培养,以在超中获得α-葡萄糖酶 (AGase).
通过量化从PNP-α-1,4-葡萄糖酸中释放的p-nitrophenol (PNP) 来测量AGase活性.
通过使用麦芽糖基质和原始AGase酶,优化了IMO的生产.

主要成果:

扎拉里亚 sp. 扎拉里亚 sp. 在Him3中,葡萄糖,曼诺糖,麦芽糖,糖糖和三糖的利用率很高.
用马尔托斯化产生IMO (异马尔托斯,潘诺斯,异马尔托斯) 和AGase活性 (0.0165U/mL).
AGase酶有效地降解了麦芽糖 (98%在72小时内),达到IMO最大度为138g/L,产量为49.5%.

结论:

扎拉里亚 sp. 扎拉里亚 sp. Him3通过其分泌的α-葡萄糖酶 (AGase) 能够产生异多糖 (IMO).
这种酵母代表了一种有价值和具有工业前景的酶资源,用于同时生产各种寡糖,包括FOS和IMO.
这些发现支持开发使用微生物酶合成寡糖的新生物技术过程.

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