从Trichoderma clade Viride中发现的新型生物.
Tamás Marik1, Bonaya Gufu2, Anusha Vishwanathula2
1Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, 6726, Szeged, Hungary. marik.tamas@szte.hu.
Natural products and bioprospecting
|August 1, 2025
概括
这项研究确定了来自Trichoderma真菌的新型菌生物,包括peptaibols和lipopeptaibols. 分子模拟显示,这些化合物倾向于在水中形成扭曲的螺旋结构.
科学领域:
- 菌类学和自然产品化学 化学
- 生物化学和结构生物学
背景情况:
- 类真菌是已知的生物活性二次代谢物,包括生物的生产者.
- 生物组,包括生物体产生的所有生物,是多样化的,在许多真菌物种中不完全表征.
研究的目的:
- 为了研究和描述Viride类的各种Trichoderma物种的peptaibiome.
- 发现和阐明新型菌生物及其结构性质.
主要方法:
- 高性能液体染色学-质谱学 (HPLC-MS) 用于生物的识别和表征.
- 加快分子动力学模拟 (aMD) 用于研究选定类生物的折叠动力学和三维结构.
主要成果:
- 鉴定了367种peptabiotics,包括216种peptaibols和111种lipopeptaibols,其中161种新型peptaibols和73种新型lipopeptaibols.
- 引入了新的peptaibol子组:斯特里戈塞林A,B和多罗托普辛A,B,以及脂泰博尔序列:利波斯特里戈塞林和利波哈马丁.
- 发现了41种具有不寻常C结末的peptaibol类化合物,并观察了peptaibols中倾向于扭曲,右手螺旋结构的倾向.
结论:
- 特里科德玛维里德 (Trichoderma Viride) 群体拥有丰富多样的生物群,产生了众多新型生物.
- 对Trikoningin KA V的结构分析表明,水性环境中的Viride clade peptaibols具有特定的结构偏好.
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