相关实验视频
Updated: Sep 8, 2025
07:02
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
7.4K
LncRNA PCED1B-AS1通过向miR-361-3p促进子宫癌的进展
Shuyu Wang1, Junwei Wang2, Ke Wang1
1Chemistry Teaching and Research Section, Henan Medical College, Zhengzhou, China.
Nucleosides, nucleotides & nucleic acids
|August 1, 2025
概括
长非编码RNAPCED1B-AS1在宫癌中高度表达,促进瘤生长,并表明预后不佳. 针对PCED1B-AS1及其与miR-361-3p的相互作用,可能为宫癌提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 子宫癌的发病率正在上升,需要新的瘤相关生物标志物.
- PCED1B-AS1 (长非编码RNA) 在宫癌进展中的作用及其潜在机制尚未完全理解.
研究的目的:
- 为了研究PCED1B-AS1在宫癌组织中的表达.
- 分析PCED1B-AS1的临床意义及其与患者预后的相关性.
- 阐明PCED1B-AS1在宫癌中的功能作用和调控机制,特别是其与miR-361-3p的相互作用.
主要方法:
- 定量逆转录聚合酶连锁反应 (qRT-PCR) 用于PCED1B-AS1表达分析.
- 临床意义的奇平方测试和考克斯回归模型.
- 恩科里数据库和露西法酶报告测试验证PCED1B-AS1和miR-361-3p的相互作用.
- 细胞计数工具-8 (CCK-8) 和Transwell测试用于评估细胞增殖,迁移和入侵.
主要成果:
- 与相邻组织相比,PCED1B-AS1在宫癌组织中显著上调.
- 高PCED1B-AS1表达与晚期瘤等级,阶段和FIGO分类相关,预测不良预后.
- PCED1B-AS1表达与miR-361-3p水平有负相关性.
- 沉默PCED1B-AS1抑制了宫癌细胞的生长,迁移和入侵,这一效应被miR-361-3p抑制部分逆转.
结论:
- 在宫癌中,PCED1B-AS1显著过度表达,其与miR-361-3p的调节轴与瘤进展有关.
- 增加PCED1B-AS1表达是宫癌的一个有价值的预后生物标志物.
- PCED1B-AS1代表了新的宫癌干预措施的潜在治疗标.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K
01:22MicroRNAs
3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:06Induced Pluripotent Stem Cells
4.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.4K
03:18Non-LTR Retrotransposons
11.8K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.8K
01:20The Retinoblastoma Gene
4.2K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K